Objective: To identify the Leishmania species being responsible for cutaneous leishmaniasis in Multan. Method: Parasites were isolated from clinically and parasitologically confirmed lesions of cutaneous leishmaniasis from 30 patients by fine needle aspiration (FNA). The bioptical materials were then cultured in Evans Tobie's medium and parasites isolated were identified by isoenzyme electropho...

both zoonotic and anthroponotic cutaneous leishmaniasis (cl) caused by l. major and l. tropica, respectively, are endemic in different parts of iran. this study was performed to investigate the new changes in epidemiological pattern of cl, and to identify the species of leishmania and the strains of l. major isolated from different endemic areas of iran. seventy-two isolates from 245 samples co...

The aim of the study was to evaluate usefulness determining markers myocardial damage, such as: aspartate aminotransferase, total creatine kinase, cardiac kinase isoenzyme, lactate dehydrogenase, dehydrogenase isoenzyme and peripheral blood lactic acid in sport horses subjected physical exertion. research carried out on 50 three breeds: Ukrainian Riding, Hanoverian Westphalian, aged 4-16 years....

In this study, we used Random Amplified Polymorphic DNA (RAPD) for identification of 17 isolates of Leishmania from the skin and reticuloendothelial system of humans, animal reservoirs (rodent and dog) and sandflies in various parts of Iran in the last decade. Fifteen species have been confirmed by isoenzyme characterization by the London School of Hygiene and Tropical Medicine and Shiraz ...

Serum lacticodehydrogenase (LDH) is commonly increased in patients with haematopoietic malignancies and has been shown to be a prognostic factor in patients with non-Hodgkin's lymphoma (NHL) and myeloma. We have examined the LDH isoenzyme content in serum of 326 patients, including 252 patients with NHL (202 at diagnosis and 50 at relapse), 28 patients with Hodgkin's disease, 17 patients with C...

We examined ventricular myosin isoenzymes in hearts of normal and thyroxine (T,)treated rabbits by polyacrylamide gel electrophoresis using non-dissociating conditions as described by Hoh et al. (1977). The rabbits received daily injections of T4 (150 /ig/kg) or saline and were killed at 1 to 17 days. A myosin-enriched fraction was prepared from each ventricle by extraction in 100 mM pyrophosph...

We present a new method for quantitative determination of alkaline phosphatase isoenzymes. This method consists of electrophoretic separation on cellulose acetate membranes, special fixation technique to avoid elution and diffusion of enzyme protein during incubation, specific staining, and quantitative evaluation by densitometric measurement. We highly recommend the precedure for routine clini...

Human isoenzyme LDH-X (lactate dehydrogenase isoenzyme X) was isolated from seminal fluid of frozen semen samples by affinity chromatography by using oxamate-Sepharose and AMP-Sepharose. In the presence of 1.6 mM-NAD+, isoenzyme LDH-X does not bind to AMP-Sepharose, whereas the other lactate dehydrogenase isoenzymes do. This is the crucial point in the isolation of isoenzyme LDH-X from the othe...