Dibutyryl cyclic AMP and forskolin inhibited the contraction induced by norepinephrine (NE) more strongly than the high K(+)-induced contraction in isolated rat aorta. These inhibitors inhibited the 45Ca2+ influx stimulated by NE but not that by high K+, and they inhibited NE-induced inositol monophosphate accumulation. These results suggest that cAMP inhibits NE-induced contraction, at least p...

The present study investigated the vasorelaxant action of the aqueous leaves extract of Persea americana on isolated rat aorta. The results showed that the extract produced significant vasorelaxation and that the effect is dependent on the synthesis or release of endothelium-derived relaxing factors (EDRFs) as well as the release of prostanoid. The extract also reduced vasoconstriction probably...

Different subtypes of GABAA (gamma-aminobutyric acid A) receptors, through their specific regional and cellular localization, are involved in the manifestation various functions, both at central peripheral levels. We hypothesized that non-neuronal receptors expressed on blood vessels, which positive allosteric modulators exhibit vasodilatory effects. This study two parts: one to determine prese...

Two novel migrated pimarane-type diterpenes named neoorthosiphols A (1) and B (2) were isolated from the water decoction of the leaves of Orthosiphon aristatus (Lamiaceae), which has been prescribed in Javanese traditional medicine (jamu) for the treatment of hypertension, etc. The absolute chemical structures have been elucidated on the basis of physicochemical properties. It has been found th...

A new indole alkaloid; neonaucline (1), along with six known compounds-Cadamine (2), naucledine (3), harmane, benzamide, cinnamide and blumenol A-were isolated from the leaves of Ochreinauclea maingayii (Rubiaceae). In addition to that of compound 1, (13)C-NMR data of cadamine (2) and naucledine (3) were also reported. Structural elucidations of these alkaloids were performed using spectroscopi...

standardization of induction of oxidative stress with fenton mixture (fm) in isolated perfused rat kidney and the antioxidant effect of terminalia arjuna bark in the isolated oxidatively stressed rat kidney has been evaluated. six groups each containing eight isolated perfused rat kidneys were used for the present study and the oxidative stress was induced by perfusing the isolated kidneys with...

it has been proposed that organic nitrates such as glyceryl trinitrate (gtn), used in the treatment of cardiovascular diseases, act by producing nitric oxide (no). however, the biochemical pathway for no formation from gtn is not well understood. in the present study, we showed that nitrate formation from gtn, by isolated rat hepatocytes, was inhibited about 50% when cellular glutathione was de...

aorta, aorta explants and subcultures grown under different conditions. J Cell Biol 75: 166-184, 1977 15. Oewald B, Touster O: A new a-mannosidase occurring in Golgi membranes. J Biol Chem 248: 7223-7233, 1973 16. Bowers WE, Finkenstaedt JT, deDuve C: Lysosomes in lymphoid tissue. I. The measurement of hydrolytic activities in whole homogenates. J Cell Biol 32: 325-337, 1967 17. Daly MM: Effect...