BACKGROUND: Herpes simplex keratitis is a sight threatening ocular infection. A rapid and specific diagnosis is essential for the institution of specific antiviral therapy and to avoid complications that can arise from misdiagnosis and inappropriate treatment. Though a variety of techniques are available, isolation of Herpes simplex virus 1 (HSV-1) in culture provides the most reliable and spec...

Singular Perturbations represent an advantageous theory to deal with systems characterized by a two-time scale separation, such as the longitudinal dynamics of aircraft which are called phugoid and short period. In this work, the combination of the NonLinear Geometric Approach and the Singular Perturbations leads to an innovative Fault Detection and Isolation system dedicated to the isolation o...

Through anther and microspore culture methods, complete homozygous plants can be produced within a year as compared to the long inbreeding method. Isolated microspore culture is one of the most important techniques for rapid development of haploid plants. The efficiency of this method is influenced by several factors such as cultural conditions, growth regulators, plant media, pretreatments, ph...

The move from hardware middleboxes to software network functions, as advocated by NFV, has proven more challenging than expected. Developing new NFs remains a tedious process, requiring that developers repeatedly rediscover and reapply the same set of optimizations, while current techniques for providing isolation between NFs (using VMs or containers) incur high performance overheads. In this p...

The methods employed for the quantitative isolation of stool bacteria in man have not been standardized. Schaedler, Dubos, and Costello have outlined in detail their methods for isolation of aerobic and anaerobic bacteria in specimens obtained from mice.' However, in vitro growth factors for the isolation of the bacterial flora of mice are not applicable at all times to man. While undertaking a...

Different techniques were compared for their effectiveness in the disruption of the rigid cell walls of Basidiomycetes. Grinding under liquid nitrogen, stirred glass bead milling and enzymatic cell lysis were applied to the mycelia of Pleurotus sapidus and Lepista irina grown submerged. Each of the disruption procedures was evaluated by testing the quantity and quality of released intracellular...

Fault detection and isolation (FDI) has become a useful strategy for determining fault appearance and on-line reconfiguration. However, unknown scenarios during on-line performance are still an open field for research. Different methods, such as knowledge-based techniques or analytical redundancy, have been followed. Nevertheless, both methods present inherent drawbacks for isolation. The prese...

A total of 33 isolates of Fusarium sp. were isolated from soil samples collected from a mangrove forest in an area in Kampung Pantai Acheh, Balik Pulau, Pulau Pinang, Malaysia. The isolates were isolated using soil dilution, direct isolation and debris isolation techniques. The debris isolation technique yielded the most isolates, with a total of 22 Fusarium isolates. Based on identification us...

We apply time-domain system identification techniques to an electromagnetic actuator. This actuator is part of the vibration isolation testbed at the University of Michigan.

The inability to achieve durable pulmonary vein isolation remains a major limitation to catheter ablation for the treatment of atrial fibrillation (AF). In this review, we discuss the research performed over the past decade investigating methods to improve lesion permanence for the goal of durable pulmonary vein isolation (PVI). Investigations evaluating procedural techniques, adjunctive pharma...