The evolution over 30 years of a population of methicillin-resistant Staphylococcus aureus (MRSA) from a tertiary referral hospital was studied by phylogenetic analysis of SmaI-generated restriction fragment length polymorphisms (RFLPs). The results suggest that a new clone of MRSA appeared at the hospital in the early 1980s, which, although usually retaining its ancestral phage-type, developed...

To define the PKD1 locus further, the gene involved in the most frequent form of adult polycystic kidney disease, probes from 16 polymorphic loci were mapped on 16p13.1-pter with the combined use of cell lines containing rearranged chromosomes and family studies. Five breakpoints in the distal part of 16p arbitrarily subdivided the loci into five groups. By analysing 58 recombination events amo...

steoporosis is a common disease in which the bones become prone to fracture as a result of loss of bone mineral density (BMD). The estrogen receptor (ER) gene is a candidate gene for osteoporosis. This study assesses the relation between estrogen receptor-α gene polymorphism and osteoporosis in a population of Iranian women. Materials & Methods: In the present study, we investigated 200 preand/...

The syndrome of multiple endocrine neoplasia type 2B (MEN 2B) resembles that of MEN 2A in that both include medullary carcinoma of the thyroid, pheochromocytoma, and autosomal dominant inheritance, but is distinct in that MEN 2B patients have neuromas of the mucous membranes. MEN2A has been linked to RBP3, D10S5, FNRB, D10S15, and D10Z1 near the centromere of chromosome 10. We examined linkage ...

Four molecular approaches to determining the types of Candida albicans strains were compared. The strains used were those whose repeated DNA (ribosomal and mitochondrial) EcoRI restriction fragment length polymorphisms (RFLP) were determined by Stevens et al. (D. A. Stevens, F. C. Odds, and S. Scherer, Rev. Infect. Dis. 12:258-266, 1990). Scherer and Stevens (S. Scherer and D. A. Stevens, Proc....

OBJECTIVES To investigate whether T-cell receptor (TCR) beta chain germline alleles, either alone or in combination with a particular HLA-genotype, are associated with rheumatoid arthritis (RA). METHODS Three restriction fragment length polymorphisms (RFLPs), detected with TCR constant (TCRBC2) and variable (TCRBV8, TCRBV11) gene segments were analysed in a representative group of Belgian, HL...

Restriction fragment length polymorphisms (RFLPs) of the X-chromosome genes hypoxanthine phosphoribosyl transferase (HPRT) and phosphoglycerate kinase (PGK) were studied in 34 female patients with primary myelodysplastic syndromes (MDS). Twelve patients (35%) were heterozygous at the HPRT or PGK loci for BamHI or BglI RFLPs, respectively. In eight patients showing PGK polymorphisms, clonality w...

The aim of this study was to subtype Listeria monocytogenes strains present in an avocado processing facility using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLPs) and pulsed-field gel electrophoresis (PFGE). The inlA gene of the L. monocytogenes isolates was PCR amplified and cut with the restriction endonuclease AluI or Tsp509I. Initial investigation of 64 L. ...

This paper outlines a PCR-based approach for population genetics that offers several advantages over conventional Southern blotting methods for revealing restriction-fragment-length polymorphisms (RFLPs) in nuclear DNA. Primers are constructed from clones isolated from a nuclear DNA library, and these primers subsequently are employed in in vitro syntheses of homologous regions. Amplified produ...

SOURCE/DESCRIPTION; A 4.5 kb (pMCT96.1) or a 3.7 kb (pMCT96.2) of Mspl fragment from cosmid MCT96 isolated by an oligonucleotide (GTGTGTGTGTGTGTGTGTGT) (1) was subcloned into the Accl site of pUC18. POLYMORPHISM: pMCT96.1 : Rsal identifies 3 systems of site polymorphisms; system 1: 4.7 kb (Rl) or 3.7 kb (R2); system 2: 3.0 (R3) kb or 2.8 kb (R4); system 3: 1.0 kb (R5) or 0.9 kb (R6) pMCT96.2 : ...