The effects of taxol on antitubulin immunofluorescent staining patterns, cellular DNA content, and labeling with [3H]thymidine were measured for the taxol-sensitive HL60 and taxol-resistant K562 cell lines after exposures for 0, 4, 12, and 24 h. Taxol caused a relative increase in the fraction of 4C interphase and metaphase cells in both lines although the 4C interphase accumulation was greater...

Many chemokine receptors are typically found on natural killer cells, including CX3CR1, the receptor for the chemokine Fractalkine (FKN). This study explored whether interaction between CX3CR1 and FKN is relevant for NK cell functions in cytotoxicity against tumors. FKN expression was examined by polymerase chain reaction and CX3CR1 expression in NK cells was analyzed by flow cytometry. NK cell...

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compounds containing triazene ring structure are cytotoxic agents and clinically used as antitumor alkylating agents. in this study, a series of triazene derivatives holding alkyl and aryl moieties were synthesized and proved to be potent cytotoxic agents in-vitro particularly against eight cancer cell lines (pc3, ht29, hela, hl60, jurkat, k562, mcf7, hepg2) and a non-cancerous cell line (huvec...

Compounds containing triazene ring structure are cytotoxic agents and clinically used as antitumor alkylating agents. In this study, a series of triazene derivatives holding alkyl and aryl moieties were synthesized and proved to be potent cytotoxic agents in-vitro particularly against eight cancer cell lines (PC3, HT29, Hela, HL60, Jurkat, K562, MCF7, HepG2) and a non-cancerous cell line (HUVEC...

Herbimycin A, a benzoquinonoid ansamycin antibiotic, is found to reduce intracellular phosphorylation by tyrosine protein kinase. The human chronic myelogenous leukemia cell line K562 expresses a structurally altered c-abl protein with tyrosine kinase activity. When K562 cells are induced to undergo erythroid differentiation by hemin, reduction in the intracellular level of tyrosine phosphoryla...

Cytotoxicity and cell growth inhibition studies were performed for five distinct cobalt(ll) [Co(2)(acac)tpmc](ClO(4))(3), [Co(2)(dibzac)tpmc](ClO(4))(3), [Co(2)(hfac)tpmc](CIO(4))(2), [Co(2)(tmhd)tpmc](CIO(4))(3) and [Co(2)(ox)tpmc](CIO(4))(2).3H(2)0 and five molybdenum(Vl) complexes, [MoO(2)(pipdtc)(2)], [MoO(2)(morphdtc)], [MoO(2)(timdtc)(2)], [MoO(2)(pzdtc)(2)] and [MoO(2)(N-Mepzdtc)(2)]. Th...

Anti-thymocyte globulin (ATG), a horse antiserum to human thymus tissue, has been shown to induce granulocytic differentiation of the III60 human leukemia cell line. In this paper we describe the effect of ATG on leukemic blasts and its effect on other human leukemia cell lines in vitro. The in vitro differentiation effect of ATG was observed in blasts from two patients with leukemia and the hu...

Background: Plants are a proven source of anti-tumor compounds and it is reasonable to assume that many such substances remain to be discovered. In Iranian folk medicine, ethanolic extract of the aerial parts of Daphne mucronata Royle is used against various skin disorders.Objective: In order to evaluate anti-neoplastic plants, we initiated a cytotoxic study using Daphne mucronata that is indig...

To explore the effects of resveratrol in a human myelogenous leukemia cell line K562 and its potential molecular mechanisms. The anti-proliferation effect of resveratrol-induced apoptosis on K562 cells were detected using MTT assay. Western blotting was performed for detecting changes of SphK1 expression in total cell protein and membrane/cytosol protein in K562 cells respectively after exposur...