BubR1 performs several roles during mitosis, affecting the spindle assembly checkpoint (SAC), mitotic timing, and spindle function, but the interdependence of these functions is unclear. We have analyzed in Drosophila melanogaster the mitotic phenotypes of kinase-dead (KD) BubR1 and BubR1 lacking the N-terminal KEN box. bubR1-KD individuals have a robust SAC but abnormal spindles with thin kine...

The anaphase-promoting complex/cyclosome (APC/C) regulates sister chromatid segregation and the exit from mitosis. Selection of most APC/C substrates is controlled by coactivator subunits (either Cdc20 or Cdh1) that interact with substrate destruction motifs--predominantly the destruction (D) box and KEN box degrons. How coactivators recognize D box degrons and how this is inhibited by APC/C re...

The role of feature correlations in semantic memory is a central issue in conceptual representation. In two versions of the feature verification task, subjects were faster to verify that a feature () is part of a concept (grapefruit) if it is strongly rather than weakly intercorrelated with the other features of that concept. Contrasting interactions between feature correlations and S...

Introduction Successful completion of chromosome segregation and cytokinesis requires the coordinated activation and inactivation of numerous mitotic regulators. Inactivation is largely brought about by ubiquitindependent proteolysis, with the relevant E3 ubiquitin ligase being the anaphase promoting complex, or cyclosome (APC/C) (Irniger et al., 1995; King et al., 1995; Sudakin et al., 1995). ...

Anaphase-promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase incorporated with Cdh1 and/or Cdc20 recognizes and interacts with specific substrates, and faithfully orchestrates the proper cell cycle events by targeting proteins for proteasomal degradation. Experimental identification of APC/C substrates is largely dependent on the discovery of APC/C recognition motifs, e.g., the D-box an...

The endoplasmic reticulum transmembrane receptor Ire1 senses over-accumulation of unfolded proteins in the endoplasmic reticulum and initiates the unfolded protein response (UPR). The cytoplasmic portion of Ire1 has a protein kinase domain (KD) and a kinase extension nuclease (KEN) domain that cleaves an mRNA for encoding the Hac1 transcription factor needed to express UPR genes. During this UP...

Mitotic progression is driven by proteolytic destruction of securin and cyclins. These proteins are labeled for destruction by an ubiquitin-protein isopeptide ligase (E3) known as the anaphase-promoting complex or cyclosome (APC/C). The APC/C requires activators (Cdc20 or Cdh1) to efficiently recognize its substrates, which are specified by destruction (D box) and/or KEN box signals. The spindl...

Serum immunoglobulin concentrations were determined on sera from 298 healthy children aged six months to six years using the Hyland laser nephelometer PDQ system. Age-specific 95% reference ranges for serum IgG, IgA and IgM are presented; considerable care has been taken to ensure statistical validity of the reference ranges. The wide range of values in children under two years suggest that mea...

The effects of parametric uncertainty in stable state space systems are analyzed by averaging the state covariance over the statistics of the uncertain parameters. For natural frequency uncertainty, this computation is related to the Fourier transform of the probability density function of the uncertain parameter. Equipartition and incoherence are illustrated for a single mode oscillator. Avera...

notion of orthogonality (related to work of Hyland and Schalk.) We develop Girard’s original theory of types, data and algorithms in our setting, and show his execution formula to be an invariant of Cut Elimination (under some restrictions). We prove Soundness Theorems of the MGoI interpretation (for Multiplicative and Multiplicative Exponential Linear Logic) in partially traced ∗-categories wi...