Chung, T. K. and Baker, D. H. 1992. Utilization of methionine isomers and analogs by the pig. Can. J. Anim. Sci. 72: 185-188. A total of 72pigs averaging 9.6 + 1.4 kg were used to evaluate the efficacy of supplemental L-methionine (Met), D-Met, DL-Met and Dl-methionine hydroxy analog free acid (DL-HMB) when added to a Met-deficient feather meal-corn-soybean meal-dried whey diet. Growth performa...

In the sand-fly mid gut, Leishmania promastigotes are exposed to acute changes in nutrients, e.g. amino acids (AAs). These metabolites are the main energy sources for the parasite, crucial for its differentiation and motility. We analysed the migratory behaviour and morphological changes produced by aliphatic, monocarboxylic, dicarboxylic, heterocyclic and sulphur-containing AAs in Leishmania a...

In this paper, we present a novel, "single experiment" methodology based on genetic engineering of metabolic pathways for direct intracellular production of non-canonical amino acids from simple precursors, coupled with expanded genetic code. In particular, we engineered the intracellular biosynthesis of L-azidohomoalanine from O-acetyl-L-homoserine and NaN3, and achieved its direct incorporati...

Reelin and glutamic acid decarboxylase (GAD)67 expressed by cortical gamma-aminobutyric acid-ergic interneurons are down-regulated in schizophrenia. Because epidemiological studies of schizophrenia fail to support candidate gene haploinsufficiency of Mendelian origin, we hypothesize that epigenetic mechanisms (i.e., cytosine hypermethylation of CpG islands present in the promoter of these genes...

L-Methionine-dl-sulfoxide can support the growth of an Escherichia coli methionine auxotroph, suggesting the presence of an enzyme(s) capable of reducing the sulfoxide to methionine. This was verified by showing that a cell-free extract of E. coli catalyzes the conversion of methionine sulfoxide to methionine. This reaction required reduced nicotinamide adenine dinucleotide phosphate and a gene...

Six ethionine-resistant (Etr) regulatory mutants of Saccharomycopsis lipolytica Sl/1 overproducing methionine have been isolated. Five of them are also resistant to seleno-methionine. The activity of homocysteine synthase (O-acetyl-L-hormoserine-acetate lyase, adding hydrogen sulfide) is derepressed in these mutants and is not susceptible to the methionine-mediated repression. The pool of free ...

The regulation of genes by amino acids is attracting increasing attention. In the present study, we investigated the restriction of expression of the class of glutathione S-transferase (GST Yp) by sulfur amino acids. Hepatocytes isolated from male Sprague-Dawley rats were cultured with L-15-based medium containing low (LSAA; 0.1 mmol/L L-methionine and 0.1 mmol/L L-cysteine) or high (HSAA; 0.5 ...

This study evaluates the effects of carbon, nitrogen, and sulfur dopants on photocatalytic activity TiO2 for degradation oxytetracycline (OTC) chemical oxygen demand (COD) removal from licorice extraction plant wastewater (LEPW). Three novel visible-light-responsive nanostructures, including L-Histidine-TiO2, L-Methionine-TiO2 L-Asparagine-TiO2, were successfully synthesized. The results showed...

L-Methionine sulfoximine is a substrate of L-amino acid oxidase (Crotalus adamanteus), glutamine transaminase, and y-cystathionase. In the reaction catalyzed by L-amino acid oxidase, methionine sulfoximine is converted to oc-imino-y-methylsulfoximinylbutyrate, which undergoes rapid y elimination yielding methane sulfinimide and 2-imino-3-butenoic acid. Methane sulfinimide is converted to methan...

Whole-blood free amino acids were measured in a control group made up of eight healthy women fasted for 12 h and also in eight patients with acute pancreatitis, five patients with acute cholecystitis and seven patients with acute appendicitis. Blood was withdrawn immediately on admission to hospital and again 3 d later following a controlled peripheral parenteral nutrition diet; this is with th...