y-Glutamyl phosphate reductase, the second enzyme of proline biosynthesis, catalyses the formation of L-glutamic acid 5-semialdehyde from y-glutamyl phosphate with NAD(P)H as cofactor. It was purified 150-fold from crude extracts of Pseudomonas aeruginosa PAO 1 by DEAE-cellulose chromatography and hydroxyapatite adsorption chromatography. The partially purified preparation, when assayed in the ...

L-Ornithine cyclodeaminase (OCD) is involved in L-proline biosynthesis and catalyzes the unique deaminating cyclization of L-ornithine to L-proline via a D-pyrroline-2-carboxyrate (Pyr2C) intermediate. Although this pathway functions in only a few bacteria, many archaea possess OCD-like genes (proteins), among which only AF1665 protein (gene) from Archaeoglobus fulgidus has been characterized a...

The physiology of Clostridium sporogenes was investigated in defined, minimal media. In batch culture, the major end products of glucose dissimilation were acetate, ethanol and formate. When L-proline was present as an electron acceptor, acetate production was strongly enhanced at the expense of ethanol. As judged by assay of the relevant enzymes, glucose was metabolized via the Embden-Meyerhof...

Proline dehydrogenase (PRODH) catalyzes the first step of proline catabolism, the flavin-dependent oxidation of proline to Delta(1)-pyrroline-5-carboxylate. Here we present a structure-based study of the PRODH active site of the multifunctional Escherichia coli proline utilization A (PutA) protein using X-ray crystallography, enzyme kinetic measurements, and site-directed mutagenesis. Structure...

1. Ornithine delta-transaminase (l-ornithine-2-oxo acid aminotransferase, EC 2.6.1.13) and Delta(1)-pyrroline-5-carboxylate reductase [l-proline-NAD(P) 5-oxidoreductase, EC 1.5.1.2] were demonstrated in fat-body and flight-muscle tissues of the silkmoth Hyalophora gloveri. Arginase (l-arginine ureohydrolase, EC 3.5.3.1) is also present in these tissues. 2. Arginase, ornithine transaminase and p...

This trail was conducted to study the effect of L-proline on the growth performance, and blood parameter in the weaned lipopolysaccharide (LPS)-challenged pigs. Thirty six pigs (9.13±0.85 kg) were assigned randomly to dietary treatments in a 2×3 factorial arrangement in a 20-d growth assay. Factors were intraperitoneal injection with saline or LPS, and three dietary L-proline supplement levels ...

The observed sensitivity of Listeria monocytogenes to the toxic proline analogue L-azetidine-2-carboxylic acid (AZ) suggested that proline synthesis in Listeria may be regulated by feedback inhibition of gamma-glutamyl kinase (GK), the first enzyme of the proline biosynthesis pathway, encoded by the proB gene. Taking advantage of the Epicurian coli mutator strain XL1-Red, we performed random mu...

freezing injury is an important limiting factor in the production of pomegranate in iran.the aim of the present study was to evaluate the effect of paclobutrazol (pbz) on cold hardiness of pomegranate (punica granatum l.) cv. ‘malas saveh’. different concentrations of pbz including 0 (control), 31, 62, 125 or 250 mg l–1 were sprayed on one-year-old plants in august and the electrolyte leakage o...

afforestation of contaminated areas is considered as a possible strategy for reduction of contaminations. in the present study, the effects of lead (pb) and cadmium (cd) were investigated on chlorophyll fluorescence parameters (fv/fm, fo, and fm), photosynthetic pigments (chlorophyll a, b, and total chlorophyll), and proline in one-year-old seedlings of celtis caucasica and robinia pseudoacacia...