We have used an indirect method to compare the dynamic properties of microtubules (MTs) in the main shaft and distal regions of the axon. Individual MTs are staggered along the length of the axon and consist of a labile domain situated at the plus end of a stable domain (Baas and Black, 1990). As a result of this organization and the plus-end-distal orientation of axonal MTs, the most distal re...

Myelin phospholipids have been examined by a separation-reaction-separation procedure for two-dimensional thin-layer chromatography on silica gel. After separation in one dimension, alk-1-enyl groups are cleaved by exposure of the plates to HCl fumes. Development in the second dimension quantitatively separates acid-labile and acid-stable phosphoglycerides as well as the aldehydes released from...

Photo-labile molecules have been widely used not only in organic synthesis but also in biological study. The chemistry of the typical photo-labile organic molecules, including their structure, mechanism underlying their photo-lability and strategies to integrate them with biomolecules, is reviewed to illustrate the structural basis for photo-activable caged compounds. Biological applications of...

a high cardiac output and a lower than normal total peripheral resistance. It was our feeling, based on this original work, that this pattern might be an early phase or a benign type of essential hypertension. This is a report of the first follow-up study done on a group of such labile hypertensive patients in whom the hemodynamics and clinical status have been studied over a period of 50 month...

Part I. The Production of Bacterial Toxins .. 4 1. The use of media of simplified composition.......................... 4 2. The possible relationship of oxidation-reduction systems to the formation of toxins............................................. 6 3. Other factors affecting the formation of toxins .......... .......... 8 4. Toxins as products of secretion, autolysis, or the action of en...

We used Salmonella enteritidis serotype dublin strain SL1438, a nonreverting, aromatic-dependent, histidine-requiring mutant, as a recipient for a recombinant plasmid coding for production of the nontoxic B subunit of the heat-labile Escherichia coli enterotoxin. The S. enteritidis derivative EL23 produced heat-labile enterotoxin subunit B that was indistinguishable from heat-labile enterotoxin...