philosophy of the counselee as principal decision maker is affirmed, and the nondirective nature of most genetic counseling is emphasized. Genetic heterogeneity as a confounding variable is stressed, and the need for correct diagnosis is emphasized. There are a number of rather dogmatic statements. Some of these, such as the opinion that every chromosome study should be a banded one and a cytog...

BACKGROUND Processing multiple tissue sections in large Mohs cases is time consuming and labor intensive. OBJECTIVE To present innovative laboratory techniques to facilitate processing of large Mohs cases. METHODS A method for processing a large dermatofibrosarcoma protuberans Mohs case is outlined. RESULTS Modifications in tissue processing and equipment employed in a large Mohs case are...

In designing automated systems for interpretation or manipulation of laboratory image data such as that derived from microphotographs, it is often the goal to perform operations that extract information about the structure of objects, and to separate and discern various objects within the data. Measurements of the events, called features, can then be calculated and used for process or statistic...

In five patients, a 46-year-old Surinamese man, a 45-year-old Dutch barkeeper, a 41-year-old woman from Eritrea, and an 18-year-old Afghan woman and her 43-year-old mother, tuberculosis was diagnosed and treated after a considerable delay. Such a late diagnosis and subsequent treatment is not uncommon for tuberculosis as the symptoms, physical examination, laboratory tests, imaging techniques a...

This paper describes the overnight Giemsa staining method for detecting mycoplasma infection of cell cultures. This simple method requires no special equipment and has practical application in most cell culture laboratories. This technique has been used conveniently to monitor stock cell cultures for mycoplasma infection over a 12-month period avoiding any contamination.

In November 1963 a cytopathic agent was isolated from a baby who died in the Royal Manchester Children's Hospital. This agent produced an unfamiliar effect in cell cultures, and further studies confirmed that this effect was unlike anything previously encountered by us. Since then five other agents, producing similar cytopathic effects in cell cultures, have been isolated in the Public Health L...

A huge number of laboratory testing techniques is known for assessing adhesion properties between bituminous materials and aggregates in laboratory. Besides simple empirical tests (usually established for binder specification and quality assurance), like Rolling Bottle Test or static immersion test, elaborate test methods are in use like contact angle measurement of either bitumen droplets on m...

Laboratory techniques Laboratory techniques in the investigation of fungal infections RJ Hay Introduction Fungal infections, mainly in the form of vaginal candidosis, are common in patients attending genitourinary medicine clinics. However, infections affecting the AIDS patient extend this range of fungal disease considerably. The mycoses in AIDS patients include some of the commoner and more i...

Yolk-sac inoculation of embryonated eggs was superseded 25 years ago by the use of cell cultures (often McCoy) for the isolation of Chlamydia trachomatis. Centrifugation of specimens onto the cell monolayers was shown to increase sensitivity, but little of late has further improved sensitivity which is at least ten-fold greater than that of eggs. However, culture is slow and labour intensive so...