BACKGROUND Probiotics are perceived to exert beneficial effects in the prevention and treatment of allergic diseases. OBJECTIVE There are conflicting data from studies as to an impact on allergic sensitization and asthma. METHODS Our prospective double-blind study randomly assigned 131 children (6-24 months old) with at least two wheezing episodes and a first-degree family history of atopic...

Exopolysaccharides (EPSs) are exocellular polymers present in the surface of many bacteria, including Lactobacillus and Bifidobacterium. The genome sequence of several strains revealed the presence of EPS-encoding genes. However, the physiological role that EPSs play in the bacterial ecology still remains uncertain. In this study, we have assessed the effect of EPSs produced by Lactobacillus rh...

Antibodies specifically reacting with platelets only in the presence of EDTA. by the platelet immunofluorescence test. were found in the serum of 20 patients with pseudothrombocytopenia due to in vitro EDTA-dependent platelet agglutination. These antibodies reacted optimally at 0-4’C. In 1 9 patients. IgG antibodies were detected; in 8 patients. 1gM or IgA antibodies were also found. In one pat...

A simple method is described for quantitative determination of lgG, IgA, 1gM, a2M, and other proteins without dilution of the sample. Strips of gelatinized cellulose acetate spread with antiserum are used as a supporting medium. The method is accurate, highly reproducible, and seems particularly suitable for routine work because it permits determination of hypo-, normal, and hyperglobulinemia o...

Serological and non-serological tests were performed in matched samples of cerebrospinalfluidand serum from236 syphilitic patients. An increased lgG or lgM index, or both, was found about70 times more often in symptomatic neurosyphilis than in latent syphilis without involvement of the central nervous system. An increased Ig index, together with a cell count >5/4., was only found in symptomatic...

Antibodies specifically reacting with platelets only in the presence of EDTA. by the platelet immunofluorescence test. were found in the serum of 20 patients with pseudothrombocytopenia due to in vitro EDTA-dependent platelet agglutination. These antibodies reacted optimally at 0-4’C. In 1 9 patients. IgG antibodies were detected; in 8 patients. 1gM or IgA antibodies were also found. In one pat...

Manual procedures are presented for determining immunoglobulins IgG, IgA, and 1gM in human serum. The three immunoglobulins are quantitated, after reaction with specific ant sera, by measuring light scattering by the antigen-antibody complexes. The procedure is standardized by use of pooled human serum with known concentrations of IgG, IgA, and 1gM. Results with the present system correlated ex...

A patient with the rare syndrome of simultaneous quinidine-induced thrombocytopenia and leukopenia was studied. A quinidine-dependent antiplatelet antibody was detected in her serum by platelet aggregometry and by indirect platelet-suspension immunofluorescence. A drugdependent antileukocyte antibody was demonstrated by leukoagglutination and by granulocyte immunofluorescence. Both antibodies w...

The association of bevacizumab and irinotecan has been shown to display a quick efficacy in low-grade glioma (LGG), but most patients relapse within months after cessation of therapy. From October 2012 to March 2014, four patients have been treated with irinotecan-bevacizumab followed by a metronomic maintenance with weekly vinblastine to try to prevent relapses. After a median follow-up of 23 ...

Three diluents were studied, to determine which is the best for the automated immunochemical measurement of specific serum proteins. Nine serum proteins (orosomucoid, a 1-antitrypsin, a2-macroglobulin, haptoglobin, transferrin, C3, lgG, IgA, and 1gM) were measured in physiological saline (9 g NaCI/liter), tris(hydroxymethyl)aminomethane buffer (0.01 mol/liter; pH 7.4), and physiological saline ...