Previous studies showed that hydrolysates of β-lactoglobulin (BLG) prepared using gastrointestinal proteases strongly inhibit dipeptidyl peptidase-IV (DPP-IV) activity in vitro. In this study, we developed a BLG-secreting Lactococcus lactis strain as a delivery vehicle and in situ expression system. Interestingly, trypsin-digested recombinant BLG from L. lactis inhibited DPP-IV activity, sugges...

Introduction Allergy to cow’s milk (CM) proteins has as major allergens caseins (60%), b-lactoglobulin (9%) and a-lactalbumin (4%). Thermal processes for milk industrialization include: pasteurization (low: 63 ° C for 30 min or High: 72 ° C for 15 seconds), UHT heating (145 ° C for 1-2s) and sterilization (115 ° C for 15 min).It’s accepted that these processes denature milk proteins in differen...

Background Aluminum hydroxide (Al(OH)3) is a widely used adjuvant for induction of experimentally Th2 responses in animals and hence has been used for examination of food protein allergenicity. Further Al(OH)3 is used as an adjuvant for subcutaneous allergy vaccines. In the present study we investigated the impact of the use of Al(OH)3 on the antibody specificity to the cow’s milk allergens b-l...

Background Atopic dermatitis is a multifactorial disease associated with elevated production of IgE and sensitization to multiple food and inhalant allergens.The aim of this study was to assess the levels of total serum IgE (IgE) and specific IgE (sp IgE ) for Dermatophagoides pteronyssinus (Dp), Blomia tropicalis (Bt), egg white, casein, alpha-lactalbumin and beta-lactoglobulin in patients wit...

The theoretical description of the adsorption proteins at liquid/fluid interfaces suffers from inapplicability classical formalisms, which soundly calls for development more complicated models. A Frumkin-type thermodynamic 2-d solution model that accounts nonidealities interface enthalpy and entropy was proposed about two decades ago has been continuously developed in course comparisons with ex...

Ligands usually bind to proteins by displacing water from the binding site. The affinity and kinetics of binding therefore depend on the hydration characteristics of the site. Here, we show that the extreme case of a completely dehydrated free binding site is realized for the large nonpolar binding cavity in bovine beta-lactoglobulin. Because spatially delocalized water molecules may escape det...

A wide variety of crystallization solutions are screened to establish conditions that promote the growth of a diffraction-quality crystal. Screening these conditions requires the assessment of many crystallization plates for the presence of crystals. Automated systems for screening and imaging are very expensive. A simple approach to imaging trace fluorescently labeled protein crystals in cryst...

Allergies to cow’s milk are very common and can present as life-threatening anaphylaxis. Consequently, food labeling legislation mandates that foods containing milk residues, including casein and/or b-lactoglobulin, provide an indication of such on the product label. Because contamination with either component independent of the other can occur during food manufacturing, effective allergen mana...

Colostrum puddings are widely popular in India as well some European countries. To prepare these desserts fresh colostrum from the first or second day of lactation is heated and flavoured by adding spices sugar. Depending on mixed with normal milk order to achieve a thinner consistency. However, no thickening agents like starch different polysaccharides need be added since, contrast drinking mi...

Abstract Purpose This paper studies the carbon footprint and water scarcity (WSF) of a milk protein, beta-lactoglobulin, produced by cellular agriculture compares this to extracted dairy protein from milk. The calculations microbially proteins were based on model hypothetical industrial-scale facility. purpose study is examine role relative in meeting future demand for more sustainably high nut...