We are concerned with the Lane-Emden problem{−Δu=upinΩ,u>0inΩ,u=0on∂Ω, where Ω⊂R2 is a smooth bounded domain and p>1 sufficiently large. Improving some known asymptotic estimates on solutions, we prove non-degeneracy local uniqueness of multi-spikes positive solutions for general domains. Our methods mainly use ODE's theory, various Pohozaev identities, blow-up analysis properties Green's funct...