A diagnostic antigen, C-antigen, was evaluated in serodiagnosis of alveolar hydatid disease by Western blotting. The C-antigen migrated diffusely to the region of 30-35 kDa in 8% polyacrylamide-SDS gel electrophoresis. We isolated this antigen from a crude metacestode extract by a simple method of phenol/chloroform extraction. The isolated antigen was resistant to boiling, proteolysis and acid ...

Larval behaviors prior to settlement are important for both dispersal and the likelihood that larvae will encounter settlement habitat. The role of endogenous factors such as larval age and size are likely to be important in determining pre-settlement behavior but are less well understood than exogenous factors. In a simple experiment we explored the role of larval age and size on pre-settlemen...

fish larvae have a high requirement of amino acid (aa) for energy production and growth. this study was aimed to increase knowledge of aa profile during feral carp larval ontogeny and estimate larval aa requirements. larvae were collected randomly at 1, 3, 7, 11, 15, 19, 26 and 33 days post hatch for growth and aa analysis. the composition of total aa changed significantly during ontogeny. the ...

A new system, the Toxorhynchites-fluorescent antibody (TFA) test in which the larvae of Toxorhynchites splendens mosquitoes were used for the detection of bluetongue virus (BTV) from Culicoides midges, was developed. Twenty-seven pools of Culicoides midges were collected from bluetongue-prone areas of Tamil Nadu by use of the light-trap and suction-trap methods. A suspension of each pool was in...

We describe how hookworms interact with their human hosts by comparing lymphocyte phenotyping, proliferative responses, and cytokine and chemokine secretion patterns in adults who are either mono-infected with Necator americanus or egg-negative controls resident in an area of high transmission in Brazil. Cellular immune responses against crude hookworm antigen extracts from different developmen...