INTRODUCTION This is an interventional prospective clinical study which was conducted to evaluate the efficacy, safety, predictability, ocular aberrations, and flap thickness predictability of Visumax femtosecond laser (FSL) compared to Moria M2 microkeratome (MK) in mild to moderate myopia. METHODS This study included 60 eyes who were divided into two groups. Thirty eyes in group (I) in whic...

PURPOSE To assess the refractive, visual acuity, and binocular results of laser-assisted subepithelial keratectomy (LASEK) for anisomyopia, anisohyperopia, and anisoastigmatia in children with various levels of amblyopia secondary to the anisometropic causes. SETTING Nonhospital surgical facility with follow-up in a hospital clinic setting. METHODS This retrospective review was of 53 childr...

PURPOSE An increase in corneal temperature has been implicated in subepithelial haze after surface photorefractive keratectomy. We aimed to determine if cooling the cornea by means of hydration between ablations and immediately after final surface ablation in PRK reduces haze. METHODS One hundred five eyes of 61 patients with myopia ranging from -6.00 to -9.75 D were included. One of the surg...

Holographic experiments are performed on a series of dual-use chromophore molecules wherein both irreversible photochromic and erasable photorefractive holographic gratings can be written in the same storage volume. At 675 nm, the chromophore undergoes a photochemical reaction leading to the creation of irreversible holographic gratings. Alternatively, at longer wavelengths, application of an e...

PURPOSE To evaluate epithelial healing and visual outcome after laser-assisted subepithelial keratectomy (LASEK) without alcohol de-epithelialization and to compare this technique to photorefractive keratectomy (PRK) in myopia. METHODS In a series of 1953 patients undergoing bilateral myopic PRK, an epithelial flap could be obtained by manual de-epithelialization in the left eye of 56 patient...

Monitoring the spatio-temporal characteristics of microcirculation is crucial for studying the functional activities of biotissue and the mechanism of disease. However, conventional methods used to measure blood flow suffer from limited spatial resolution or the injection of exogenous substances or the need of scanning to obtain the dynamic of regional blood flow. Laser speckle imaging (LSI) te...

For laser-assisted in situ keratomileusis (LASIK) retreatments with a previous unsuccessful mechanical microkeratome-assisted surgery, some surgical protocols have been described as feasible, such as relifting of the flap or the creation of a new flap and even the change to a surface ablation procedure (photorefractive keratectomy (PRK)). This case shows the use of femtosecond technology for th...

Laser micromachining by ablation is an established technique for the production of 2.5D and 3D features in a wide variety of materials. Mask projection techniques using excimer lasers have been used to fabricate microstructures on large panels where diamond turning and reflow techniques have reached their limits. We have developed 3D structuring tools based upon UV laser ablation of polymers to...

Laser Speckle Contrast Imaging (LSCI) has become a widely used technique to image cerebral blood flow in vivo. However, the quantitative accuracy of blood flow changes measured through the thin skull has not been investigated thoroughly. We recently developed a new Multi Exposure Speckle Imaging (MESI) technique to image blood flow while accounting for the effect of scattering from static tissu...

The ability to reliably analyze cellular and molecular profiles of normal or diseased tissues is frequently complicated by the inherent heterogeneous nature of tissues. Laser Capture Microdissection (LCM) is an innovative technique that allows the isolation and enrichment of pure subpopulations of cells from tissues under direct microscopic examination. Material obtained by LCM can be used for ...