Introduction: Theranostic nanoparticles, which integrate both diagnostic and therapeutic capabilities into one nanoagent, has great promise to ablate more effective tumoral tissue by optimizing and real-time monitoring treatment interventions, as well as monitoring therapeutic response to corresponding effect. Multifunctional theranostic nanoagent based on graphene quantum dots...

In this chapter, coherent effects that accompany the propagation of laser radiation in tissues and the interaction of laser radiation with cell flows are considered. These effects include diffraction, formation of speckle structures, interference of speckle fields, scattering from moving particles, etc. Principles of quasi-elastic light scattering (QELS) spectroscopy, diffusion wave spectroscop...

Probe-based confocal laser endomicroscopy is an emerging imaging modality that enables visualization of histologic details during endoscopy and surgery. A method of guiding the probe with millimeter accuracy is required to enable imaging in all regions of the abdomen accessed during laparoscopy. On the basis of a porcine model of laparoscopic liver resection, we report our experience of using a...

In autodyne interferometry, the beating between the reference beam and the signal beam takes place inside the laser cavity and therefore the laser fulfills simultaneously the roles of the emitter and the detector of photons. In these conditions, the laser relaxation oscillations play a leading role, both in the laser quantum noise that determines the signal-to-noise ratio (SNR) and also in the ...

The chemical shift sensitivity and significant signal enhancement afforded by laser-polarized 129Xe have motivated the application of 129Xe NMR to biological imaging and spectroscopy. Recent research done by our group has used laser-polarized 129Xe in biomolecular assays that detect ligand-binding events and distinguish protein conformations. The successful application of unfunctionalized and f...

A new microscopy technique is introduced, image scanning microscopy (ISM), which combines conventional confocal-laser scanning microscopy with fast wide-field CCD detection. The technique allows for doubling the lateral optical resolution in fluorescence imaging. The physical principle behind ISM is similar to structured illumination microscopy, by combining the resolving power of confocal-lase...

Micromirrors fabricated by MEMS technology have demonstrated to be important sensing or actuating components in many industrial and biomedical applications such as laser scanning displays, optical switch matrices, and biomedical imaging systems.Micromirrors fabricated by MEMS technology have demonstrated to be important sensing or actuating components in many industrial and biomedical applicati...

We have analyzed the image formation and dynamic properties in laser speckle imaging (LSI) both experimentally and with Monte Carlo simulation. We show for the case of a liquid inclusion that the spatial resolution and the signal itself are both significantly affected by scattering from the turbid environment. Multiple scattering leads to blurring of the dynamic inhomogeneity as detected by LSI...

The real-space dynamics in a model system of colloidal hard spheres was studied by means of time-resolved fluorescence confocal scanning microscopy. Direct experimental evidence for the presence of dynamical heterogeneities in a dense liquid was obtained from an analysis of particle trajectories in two-dimensional slices of the bulk sample. These heterogeneities manifest themselves as a non-Gau...