An efficient In vitro protocol was established for callogenesis from various plant parts (Leaf, stem, flower bud and tendril) of Passiflora vitifolia Kunth. On Murashige Skoog (MS) basal medium supplemented with concentration auxins (NAA, IAA, 2,4-D) cytokinins (BAP Kn ). Different concentrations (0.5 to 4 mg/L), IAA 2mg/L), NAA 2 mg/l) cytokinin BAP mg/L) were assessed callus induction. Growth...

Lisianthus (Eustoma grandiflorum L.) is a very popular, ornamental plant. Due to low germination of its seeds, usual techniques for its propagation are not efficient. The present study was aimed to establish callus from explants of different plant parts of Eustoma grandiflorum L. Raf Shinners in B5, LS and MS media with modifications.  The results showed that among different plant parts, leaf e...

The paper presents protocol for rapid, reliable and high frequency regeneration of plant via the culture of leaf explants in Thevetia peruviana (Pers.) Schum. In vivo leaves cultured on MS medium supplemented with a combination of 2,4-D (2.5 mg/l) and Kn (1.2 mg/l) produced stock callus after 20-28 days of inoculation. The obtained callus was dark green and friable. The small pieces of stock ca...

In order to enhance the system of potato transformation and further regeneration, four potato cultivars were transformed using the Agrobacterium tumefaciens harboring â-glucuronidase (GUS) gene.The results revealed that the highest percentages of shoot formation obtained were for Spunta cv. Stem explants resulted in more callus and regeneration after transformation and selection than leaf expla...

the jatropha curcas l., a plant belongs to euphorbiaceae family, which has great economic and medicinally value. there is high amount of oil in seeds of this plant. because of low germination ability of seed, propagation of jatropha curcas via seed is not usual. the purpose of this work is optimizing of jatropha curcas tissue culture for optimization of callus formation and propagation through ...

Verbesina encelioides belongs to the Asteraceae family and also known as golden crown beard wild sunflower. In present study callus was raised from leaf explant of V. encelioides. Callus obtained on MS medium. The used for antifungal activity identification. in-vitro anti-fungal Candida albicans gave most effective response out all studied fungi, with best being displayed in methanol extract fo...

The biotechnological potential of pectinolytic fungi isolated from citrus fruits was studied. Seventy five fungal isolates were isolated from decayed citrus fruits. All of these isolates were screened for their pectinolytic activity. Thirty six out of seventy five isolates proved to have pectinolytic activity. These isolates were identified and classified into twenty strains belonging to eleven...