Objectives : The purpose of this study was to evaluate the mutagenic potential fluoxetine and fluoxetine-galactomannan. Methods Chromosomal aberration test Salmonella typhimurium/microsome mutagenicity assay. Results results showed that (250 μg/mL) can cause chromosomal breaks treated leukocytes increase frequency reversion tester strains S. typhimurium / microsome assay only at highest concent...

Objective: Blood is a body fluid that plays role in transporting oxygen, nutrients, metabolic waste, and serves as component of the immune system. Its measurable indices such hematological profile affected by food consumed. This study aimed to examine linseed use complete feed on hematology value sheep blood. Methods: research was conducted at “Mitra Karya Farm” Purworejo Village, Gemolong Dist...

The aim of the study was to prove the effect of muramyldipeptide and lipopolysaccharide on the expression of CD14 and CD44 during an induced inflammatory response of the mammary gland and its resolution. The purpose was to clarify whether the CD14 and CD44 expression is controlled by the mechanisms of resolution. The CD44 had previously been judged to be an activation marker along with CD11b on...

Shiga toxin (Stx) binding sites in porcine tissues and leukocytes were identified by the use of Stx overlay and anti-CD77/Gb3 immunoassays. Stx1 and Stx2 bound to similar tissue locations and leukocytes, although some differences were noted. Previously unreported Stx binding sites were identified in kidney tubules, intestinal lymphoid aggregates, sinusoidal liver cells, alveolar macrophages, an...

Thyroxine (T(4)) and triiodothyronine (T(9)) are rapidly degraded by a purified preparation of myeloperoxidase (MPO) and H(2)O(2) with the formation of iodide and material which remains at the origin on paper chromatography. Deiodination by MPO and H(2)O(2) occurs more readily at pH 7.0 than at pH 5.0 in contrast to iodination by this system which is known to occur more readily at pH 5.0 than a...

The role of superoxide anion- and myeloperoxidase-dependent reactions in the light emission by phagocytosing polymorphonuclear leukocytes has been investigated using leukocytes that lack myeloperoxidase, inhibitors (azide, superoxide dismutase), and model systems. Our earlier finding that oxygen consumption, glucose C-1 oxidation, and formate oxidation are greater in polymorphonuclear leukocyte...

Blood vessels and blood cells are under continuous fluid shear. Studies on vascular endothelium and smooth muscle cells have shown the importance of this mechanical stress in cell signal transduction, gene expression, vascular remodeling, and cell survival. However, in circulating leukocytes, shear-induced signal transduction has not been investigated. Here we examine in vivo and in vitro the c...

Acid hydrolase activities were measured in separated lymphocytes, polymorphonuclear leukocytes, and the total leukocyte population of normal and tumor-bearing rats. Only aryl sulfatase activity of the lymphocytes from tumor-bearing rats was significantly increased. Those showing significant decreases in tumor bearers were cathepsin in polymorpho nuclear leukocytes and ß-galactosidaseand acid p...

Bone marrow cells and peripheral blood leukocytes from leukemic AKR/J mice synthesize sterols from acetate at a vastly greater rate than the respective cells from normal AKR/J mice, i.e., approximately 20and 100-fold. The controlling enzyme of sterol synthesis, 3-hydroxy-3-melhylglutaryl coenzyme A reducÃ-ase, is readily detectable in gradient-purified leukocytes from leukemic mice, whereas nor...

Marmoset blood leukocytes transformed in vitro by Epstein-Barr virus regularly release extracellular infectious Epstein-Barr virus with high titers of transforming activity. By comparison, human umbilical cord leukocytes and adult human leukocytes transformed by Epstein-Barr virus release either no extracellular infectious virus or small amounts, irregularly.