نتایج جستجو برای: ligases
تعداد نتایج: 3608 فیلتر نتایج به سال:
Bacteriophage-T4 and human type I DNA ligases were found capable of self-adenylating upon exposure to both riboand deoxyribo-[a-35S]thio-ATP. However, the joining reaction does not take place in the presence of the deoxyribotriphosphates. Enzyme adenylation is reversed in all cases by an excess of PP1, but the rate of reversion is lower with thio derivatives. Therefore thio derivatives can be u...
Melanoma antigen L2 (MAGE-L2) belongs to the approximately 60 gene MAGE family, which is divided into two subsets based on chromosomal location and expression pattern. MAGE proteins act as ubiquitin ligase regulators in conjunction with E3 RING ubiquitin ligases to form MAGE-RING ligases (MRLs). MAGEL2 has elevated expression in the hypothalamus and functions within a multi-subunit protein comp...
To explore if DNA linkers with 5'-hydroxyl (OH) ends could be joined by commercial T4 and E. coli DNA ligase, these linkers were synthesized by using the solid-phase phosphoramidite method and joined by using commercial T4 and E. coli DNA ligases. The ligation products were detected by using denaturing PAGE silver stain and PCR method. About 0.5-1% of linkers A-B and E-F, and 0.13-0.5% of linke...
NleG homologues constitute the largest family of Type 3 effectors delivered by pathogenic E. coli, with fourteen members in the enterohaemorrhagic (EHEC) O157:H7 strain alone. Identified recently as part of the non-LEE-encoded (Nle) effector set, this family remained uncharacterised and shared no sequence homology to other proteins including those of known function. The C-terminal domain of Nle...
1 Institute for Manufacturing, University of Cambridge, 17 Charles Babbage Rd, Cambridge, CB3 4 0FS, UK 5 2 IMEC, Kapeldreef 75, 3001 Heverlee, Belgium, Department of Mechanical Engineering, Katholieke 6 Universiteit Leuven, 3001 Leuven, Belgium 7 3 Mechanosynthesis Group, Department of Mechanical Engineering, University of Michigan, 2350 8 Hayward Street, Ann Arbor, Michigan 48109 USA 9 4 Mech...
Carbon nanotube conducting arrays were constructed via consecutive amidation reactions with the aid of a linker molecule and a condensation agent on a patterned amine-terminated glass substrate. The electrical resistivity of the nanotube films was sensitive to the degree of coverage for the substrate, making it possible to tailor nanotube multilayers suitable for use in micro- or nanoscale elec...
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