Light-sheet fluorescence microscopy enables rapid 3D imaging of biological samples. Unlike confocal and two-photon microscopes, a light-sheet microscope illuminates the focal plane with an objective orthogonal to detection axis images it in single snapshot. Its combination high contrast minimal sample exposure make ideal image thick samples sub-cellular resolution. To uniformly illuminate wide ...

Plane and crossed polarization are the two standard light modes in polarized light microscopy that are widely used to characterize crystalline and amorphous phases in cement-based materials. However, the use of the crossed polarized light mode has been found to be restrictive for studying birefringent phases quantitatively due to the extinction phenomenon that arises depending on the crystal or...

Biological specimens are intrinsically three dimensional; however, because of the obscuring effects of light scatter, imaging deep into a tissue volume is problematic. Although efforts to eliminate the scatter by "clearing" the tissue have been ongoing for over a century, there have been a large number of recent innovations. This Review introduces the physical basis for light scatter in tissue,...

We examined the Arctic bacterium Colwellia psychrerythraea strain 34H for motility at temperatures from -1 to -15 degrees C by using transmitted-light microscopy in a temperature-controlled laboratory. The results, showing motility to -10 degrees C, indicate much lower temperatures to be permissive of motility than previously reported (5 degrees C), with implications for microbial activity in f...

In light microscopy, colour CCD cameras are now capable of generating image data sets that contain more information than can be captured with slow 35 mm colour reversal film. The resolution of colour CCD cameras with a high density of sensor elements (> or = 3300 x 2200 per channel of colour) is equivalent to that of slow 35 mm colour film over typical fields of view for objectives with a wide ...