BACKGROUND We studied the oxidation of an oil rich in docosahexaenoic acid (DHA; DHASCO((R))) in lactating mothers receiving a dietary DHA supplement or a placebo. The results were compared with the oxidation of linoleic acid. METHODS Breast-feeding mothers received a dietary supplement (DHASCO; 200 mg DHA/day, n = 5) or a placebo (n = 5) for 14 days. Six weeks post partum all 10 mothers rece...

Hidden blood loss typically occurs following total hip arthroplasty (THA) and total knee arthroplasty (TKA) and is thought to be related to free fatty acid (FFA). To study the effect of linoleic acid on red blood cells and to examine the pathogenesis of hidden blood loss in vivo, we generated an animal model by injecting linoleic acid into the tail veins of rats. We collected blood samples and ...

Cystic fibrosis (CF) is associated with fatty acid alterations characterized by low linoleic and docosahexaenoic acid. It is not clear whether these fatty acid alterations are directly linked to cystic fibrosis transmembrane conductance regulator (CFTR) dysfunction or result from nutrient malabsorption. We hypothesized that if fatty acid alterations are a result of CFTR dysfunction, those alter...

We report fatty acid composition in perirenal and buttock adipose tissue in 714 deceased black and white men aged 25-44 yr in New Orleans. Percent saturated fatty acids were higher (p less than 0.001) whereas percent monounsaturated fatty acids were lower (p less than 0.001) in perirenal than in buttock fat. Percent linoleic acid was similar in both races. We conclude that dietary intake of lin...

Polyunsaturated fatty acids (PUFAs) have anti-inflammatory effects at low concentrations; however increased dietary consumption may conversely increase susceptibility to oxidation by free radicals. The objective of this study was to determine the effects of PUFAs on selective oxidative injury and inflammatory biomarkers in canine urine and serum. Dogs (n = 54) consumed a diet supplemented with ...

In the present study, the cytotoxicity of palmitic, stearic, oleic, linoleic, arachidonic, docosahexaenoic and eicosapentaenoic acids on a macrophage cell line (J774) was investigated. The induction of toxicity was investigated by changes in cell size, granularity, membrane integrity, DNA fragmentation and phosphatidylserine externalization by using flow cytometry. Fluorescence microscopy was u...

Extracts that were obtained with solvents of increasing polarity (hexane, dichloromethane, methanol, 80% and water) from the red macroalga Gracilaria domingensis evaluated by reducing power ferric reduction antioxidant (FRAP) Folin–Ciocalteu (FC) assays, lipid peroxidation inhibition β-carotene-linoleic acid assay, metal chelating ability based on iron-ferrozine system. The highest capacity was...

I. In Expt I , four adult wether sheep were given diets of hay or dried grass in an alternating sequence of feeding treatments. Each treatment period lasted for 20 days and blood samples were taken from the sheep on the last day of each period. When the diet of hay (linoleic: linolenic acid ratio 1.40) was replaced by one of dried grass (1inoleic:linoltnic acid ratio 0.28), there was an increas...

We have investigated the extent to which modifications in the essential fatty acid content of mammalian cells can affect prostaglandin production. Swiss mouse 3T3 cells stimulated with the calcium ionophore A23187 produced 1.7 to 7 times more prostaglandin E(2) (PGE(2)) when the cultures were supplemented with linoleic acid. Increases in PGE(2) production as a result of linoleic acid supplement...

Lack of available water in arid and semi-arid regions has a negative effect on the production of soybean (Glycine max L.). Nano zinc chelate (ZnO) has the potential to alleviate this issue through overcoming zinc deficiency and thereby improve plant function. Therefore, to evaluate the effectiveness of ZnO application on soybean in drought stress conditions, a split-plot experiment based on ran...