In the course of the lipoxygenase-catalyzed transformation of linoleic acid to 135-hydroperoxy-9Z,ll£'-octadecadienoic acid, iron ions are liberated. This iron release has been de­ termined using a spectrophotometric assay based on the complexation of ferrous iron by 3-(2-pyridyl)-5,6-bis-(4-phenylsulfonic acid)-l,2,4-triazine disodium salt (ferrozine). Further comparative measurements demonstr...

BACKGROUND Paraquat (PQ) has been implicated as a risk factor for the Parkinson disease phenotype (PDP) in humans and mice using epidemiologic or experimental approaches. The toxicokinetics (TK) and toxicodynamics (TD) of PQ in the brain are not well understood. OBJECTIVES The TK and TD of PQ in brain were measured after single or repeated doses. METHODS Brain regions were analyzed for PQ l...

Because taurine alleviates ethanol- (EtOH-) induced lipid peroxidation and liver damage in rats, we asked whether exogenous taurine could alleviate EtOH-induced oxidative stress in chick embryos. Exogenous EtOH (1.5 mmol/Kg egg or 3 mmol/Kg egg), taurine (4 μmol/Kg egg), or EtOH and taurine (1.5 mmol EtOH and 4 μmol taurine/Kg egg or 3 mmol EtOH and 4 μmol taurine/Kg egg) were injected into fer...

Cold acclimation of ectotherms results typically in enhanced oxidative capacities and lipid remodeling, changes that should increase the risk of lipid peroxidation (LPO). It is unclear whether activities of antioxidant enzymes may respond in a manner to mitigate the increased potential for LPO. The current study addresses these questions using killifish (Fundulus heteroclitus macrolepidotus) an...

The goal of these studies was to examine the effect of lipid peroxidation (LPO) on the function of surfactant protein A (SP-A). First, the optimal dialysis conditions for quantitative removal of EDTA and redoxactive metals from reagents were established. Surfactant phospholipids were incubated with free radical generators in the absence or presence of the SP-A or with BSA as a control. We found...

BACKGROUND Doxorubicin (DOX), an anthracycline antibiotic, is a widely used anticancer agent. In spite of its high antitumor efficacy, the use of DOX in clinical chemotherapy is limited due to diverse toxicities, including gonadotoxicity. OBJECTIVE We investigated the protective effect of nano-zinc oxide (nZnO) as an established antioxidant on DOX-induced testicular disorders. MATERIALS AND...

BACKGROUND Pulmonary damage resulting from lipid peroxidation is a principal effect of paraquat intoxication. The host-defense functions of surfactant are known to be mediated by the surfactant proteins A and D (SP-A and SP-D, respectively). The primary objective of this study was to evaluate the variations over time in levels of surfactant protein and lipid peroxidation (LPO) in lung tissue fo...

Objective: Detecting the level of antioxidant and tissue damage that can occur in liver induced metabolic syndrome by a high fructose diet rats changes after exercise and/or caloric restriction. Materials Methods: Sprague-Dawley male were divided into five groups: control (C), (M), with (ME), restriction (MCR), (MECR). To induce syndrome, 10% solution was given to drinking water for 3 months. E...

We aimed to investigate the extent of alterations in pro/antioxidant balance blood patients with relapsing–remitting multiple sclerosis (RRMS) relation drug-modified therapy, gender, disability score, and disease duration. 161 (67 men 94 women, aged 24–69 years, median 43.0) 29 healthy individuals (9 20 25–68 41.0) were included study. measured activity superoxide dismutase (SOD), glutathione p...

Etheno (ε) DNA base adducts are highly mutagenic lesions produced endogenously via reactions with lipid peroxidation (LPO) products. Cancer-promoting conditions, such as inflammation, can induce persistent oxidative stress and increased LPO, resulting in the accumulation of ε-adducts in different tissues. Using a recently described fluorescence multiplexed host cell reactivation assay, we show ...