Background—Here, we aim to identify defects of apolipoprotein (apo) B lipoprotein metabolism that characterize hypertriglyceridemia, focusing on apoC-III and apoE. Methods and Results—We studied the transport of plasma apoB within 21 distinct subfractions as separated by anti–apoC-III and anti–apoE immunoaffinity chromatography and ultracentrifugation in 9 patients with moderate hypertriglyceri...

Hepatocytes obtained from rats fed a choline-deficient diet for 3 days were cultured in a medium +/- choline (100 microM) or methionine (200 microM). We investigated how choline deficiency affected hepatic lipogenesis, apolipoprotein synthesis, and lipoprotein secretion. The mass of triacylglycerol and phosphatidylcholine secreted was increased about 3-fold and 2-fold, respectively, by the addi...

OBJECTIVE The kinetic abnormalities of apolipoprotein B (apoB)-containing lipoproteins in abdominally obese insulin-resistant individuals remain poorly understood. To determine the influence of insulin resistance, linked with abdominal obesity, on apoB metabolism at an early stage, we performed a stable isotope kinetic study of apoB in very low density lipoproteins (VLDLs), intermediate density...

We have developed a procedure for labeling lipoproteins with the fluorescent probe 3,3'-dioctadecylindocarbocyanine (Dil) and have used Dil-labeled native and acetoacetylated lipoproteins to differentiate macrophages from fibroblasts in mixed cell culture. Lipoproteins labeled with this probe were suitable for the direct viewing of their binding and internalization by cells in vitro. The labeli...

The paper analyzes the IR spectra of blood plasma from healthy donors, patients with brain infarction and coronary heart disease (CHD). It was found that structure main transport protein – albumin in infarction, CHD representatives control group is almost identical. shown absence changes secondary tertiary albumin, increase binding anionic hydrophobic metabolites dyslipidemia compared to due st...

account for only about 3% of the cholesterol accumulation in the Ehrlich cells. Since the peritoneal fluid in which the tumor cells grow is rich in very-low-density lipoproteins (VLDL), we wished to determine whether the cholesterol contained in these VLDL might be available for utilization by the cells. During incubation in vitro at 37°,the Ehrlich cells took up large quantities of cholestero...

The effects of chylomicrons, VLDL (very low density lipoprotein), HDL (high density lipoprotein), and its subfractions HDLz and HDLs, on lecithin: cholesterol acyltransferase reaction in total plasma have been studied. Plasma cholesterol esteritication was measured by the decrease in unesterified cholesterol which was determined by gas-liquid chromatography. Addition of HDL or HDLI had no effec...

The effects of chylomicrons, VLDL (very low density lipoprotein), HDL (high density lipoprotein), and its subfractions HDLz and HDLs, on lecithin: cholesterol acyltransferase reaction in total plasma have been studied. Plasma cholesterol esteritication was measured by the decrease in unesterified cholesterol which was determined by gas-liquid chromatography. Addition of HDL or HDLI had no effec...

The effect of lipid transfers on the structure and composition of high density lipoproteins (HDL) has been studied in vitro in incubations that contained the lipoprotein-free fraction of human plasma as a source of lipid transfer protein. These incubations did not contain lecithin:cholesterol acyltransferase activity and were not supplemented with lipoprotein lipase. Incubations were performed ...

Lipids such as triacylglycerols, free and esterified cholesterol and phospholipids are essentially insoluble in water; lipoproteins, which are complex macromolecules of pseudomicellar structure, have evolved to ensure their efficient transport in the vascular system of vertebrates. Plasma lipoproteins are operationally classified according to their relative lipid and protein contents and thus a...