BACKGROUND Coptis chinensis Franch is a traditional Chinese medical herb. OBJECTIVE In this article, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to rapidly, qualitatively, and comprehensively identify the components in Coptis chinensis Franch. MATERIALS AND METHODS Chromatographic separation was achieved on an Agilent Zorbax RRHD...

238 / 250 Introduction: 453 / 750 Discussion: 1478 / 1500 Nonstandard abbreviations used are: Clint,app apparent intrinsic clearance; CYP, cytochrome P450; FMO, flavin-containing monooxygenase; HLM, human liver microsomes; HPLC, highperformance liquid chromatography; KM, Michaelis Menten constant; LC-MS, liquid chromatography mass spectrometry; MS, mass spectrometry; NMR, nuclear magnetic reson...

In a growing number of laboratories the technique of liquid chromatography-tandem mass spectrometry is used for the quantification of cyclosporin A in whole blood, employing cyclosporin D as the internal standard. Cyclosporin A is extensively metabolized in vivo; in liquid chromatography-tandem mass spectrometry respective metabolites can give rise to both parent and product ions that are isoba...

A tadalafil analogue was detected in a dietary supplement marketed for tonic effect, along with hydroxyhomosildenafil and aminotadalafil. The tadalafil analogue was isolated by preparative thin layer chromatography (TLC) and its structure was elucidated using high-performance liquid chromatography (HPLC), liquid chromatography electrospray ionization-mass spectrometry (LC-ESI-MS), Fourier trans...

Extracts from a peat, a low-rank coal and a bituminous oíl shale obtained with liquid carbon dioxide were studied by capillary gas chromatography-mass spectrometry and compared with those obtained by Soxhlet extraetion with n-hexane. Although the extraetion yields with liquid carbon dioxide were lower, the composition of the extraets, comprising mainly a large variety of linear and cyclic hydro...

Liquid chromatography-mass spectrometry is one of the most powerful methods for the identification and detection of chemical structures of lipids. In this study, we attempted to identify the chemical structures of glucosylceramides from maize, rice, mushroom (maitake) and sea cucumber by liquid chromatography-ion trap mass spectrometry. For structural analysis of glucosylceramides, [M+H]+, [M+H...

UNLABELLED Current tools for liquid chromatography and mass spectrometry for metabolomic data cover a limited number of processing steps, whereas online tools are hard to use in a programmable fashion. This article introduces the Metabolite Automatic Identification Toolkit (MAIT) package, which makes it possible for users to perform metabolomic end-to-end liquid chromatography and mass spectrom...

A major protein-bound ligand in the serum of chronic hemodialysis patients was isolated from heat-deproteinized uremic serum by "high-performance" liquid chromatography (HPLC). The isolated compound was identified as 3-carboxy-4-methyl-5-propyl-2-furanpropionic acid by use of liquid secondary-ion mass spectrometry and gas chromatography-mass spectrometry. On HPLC, an authentic sample of this co...

Glyphosate is widely used in herbicides and is toxic to organism. Therefore accurate detection of glyphosate content is important for public health and food security. In this paper, methods such as gas chromatography (GC), high performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC/MS), liquid chromatography-mass spectrometry (LC/MS), ion chromatography (IC), capilla...

Methodology for determining amino acid sequences of proteins by tandem mass spectrometry is described. The approach involves enzymatic and/or chemical degradation of the protein to a collection of peptides which are then fractionated by high-performance liquid chromatography. Each fraction, containing as many as 10-15 peptides, is then analyzed directly, without further purification, by a combi...