OBJECTIVE To measure sperm DNA integrity values before and after varicocelectomy in patients with elevated preoperative levels of sperm DNA fragmentation. DESIGN Retrospective. SETTING Private urology clinic. PATIENT(S) Eleven patients with grade 1, 2, or 3 varicocele. INTERVENTION(S) Varicocelectomy. MAIN OUTCOME MEASURE(S) Sperm DNA fragmentation values were assessed before and afte...

Bull sperm that had been extracted with 0.2% Triton X-100 could be reactivated with ATP, and their movement closely resembled the motion of intact live sperm. Their motility required the presence of ATP, magnesium, and a medium of suitable salt concentration and pH. When Triton-extracted bull sperm were digested breifly with trypsin at pH 9.0, they appeared to reatin most of their normal struct...

BACKGROUND Protein tyrosine phosphorylation (TP) of human sperm is related to sperm capacitation and zona pellucida (ZP) binding. The aim of this study was to determine whether the TP of capacitated sperm is a useful marker for the ability of sperm to bind to the ZP and undergo the ZP-induced acrosome reaction (AR). METHODS Semen samples were obtained from 115 subfertile men with sperm count ...

Sperm retrieval techniques form an integral part of the assisted reproductive programme. The success of sperm separation is measured by the number of motile sperm retrieved from a given semen sample. The influence of various technical procedures and diluent media upon human spermatozoa has been tested in vitro (Jeulin et al., 1982). The percentage and velocity of motile spermatozoa are often me...

Sperm banking can preserve male fertility effectively, but the current conditions of sperm cryopreservation in China have not been investigated. This retrospective investigation was based on data collected at multiple centres in China from January 2003 to December 2008. The collected data included urogenital history, indication for cryopreservation, semen parameters, use rate, type of assisted ...

Our objective was to establish which is the best sperm retrieval technique in non-obstructive azoospermia based on the available evidence. To date, no randomized controlled trial has compared the efficiency of these strategies and thus current recommendations are based on cumulative evidence provided by descriptive, observational and controlled studies. Three outcome measures were assessed for ...

Introduction The reproductive efficiency of the breeding herd depends upon the fertility of the boar. Male fertility is essential since the boar's DNA is the primary mechanism through which genetic improvements can efficiently be accomplished. In production settings, the critical event, which begins the reproductive process, is the meeting of sperm and eggs inside the female reproductive tract....