نتایج جستجو برای: loop mediated isothermal amplification assay
تعداد نتایج: 787341 فیلتر نتایج به سال:
west nile virus (wnv) is an important zoo-notic agent having a wide host range. due to its emergence with increased virulence in a wide geographical range, its monitoring becomes im-perative. development of more rapid and sensitive molecular techniques for instance reverse trans-criptase-polymerase chain reaction (rt-pcr), reverse transcription loop-mediated isothermal amplification (rt-lamp) a...
Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...
We developed a loop-mediated isothermal amplification (LAMP) method to detect Bordetella pertussis infection. This LAMP assay detected B. pertussis with high sensitivity, but not other Bordetella species. Among nasopharyngeal swab samples from subjects with suspected pertussis, LAMP results showed a high level of agreement with results of conventional PCR. This method is a rapid, sensitive, and...
Title: Performance of Loop-Mediated Isothermal Amplification assay in the diagnosis of pulmonary tuberculosis in a high prevalence TB/HIV rural setting in Uganda Authors: Lydia Nakiyingi ([email protected]) Prossy Nakanwagi ([email protected]) Jessica Briggs ([email protected]) Tifu Agaba ([email protected]) Fank Mubiru ([email protected]) Mark Mugenyi ([email protected]) Wi...
BACKGROUND Nucleic acid tests that can simultaneously detect multiple targets with high sensitivity, specificity, and speed are highly desirable. To meet this need, we developed a new approach we call the isoPCR method. METHODS The isoPCR method is a 2-stage nested-like nucleic acid amplification method that combines a single multiplex preamplification PCR with subsequent distinct detection o...
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