نتایج جستجو برای: loop mediated isothermal amplification method lamp

تعداد نتایج: 2179620  

Journal: :Journal of clinical microbiology 2013
James Mahony Sylvia Chong David Bulir Alexandra Ruyter Ken Mwawasi Daniel Waltho

Rapid isothermal amplification methods have recently been introduced, and some of these methods offer significant advantages over PCR. The objective of this study was to develop a rapid and sensitive multiplex loop-mediated isothermal amplification (M-LAMP) assay for the detection of respiratory syncytial virus subgroups A and B (RSV A and B). We designed six primers each for the matrix gene of...

2015
Amy Creecy Patricia K. Russ Francesca Solinas David W. Wright Frederick R. Haselton Pedro V. Baptista

In this study, we integrated magnetic bead-based sample preparation and isothermal loop mediated amplification (LAMP) of TB in a single tube. Surrogate sputum samples produced by the Program for Appropriate Technology in Health containing inactivated TB bacteria were used to test the diagnostic. In order to test the sample preparation method, samples were lysed, and DNA was manually extracted a...

Journal: :Journal of clinical microbiology 2010
Yee Ling Lau Puviarasi Meganathan Parthasarathy Sonaimuthu Girija Thiruvengadam Veeranoot Nissapatorn Yeng Chen

Loop-mediated isothermal amplification (LAMP), a rapid nucleic acid amplification method, was developed for the clinical diagnosis of toxoplasmosis. Three LAMP assays based on the SAG1, SAG2, and B1 genes of Toxoplasma gondii were developed. The sensitivities and specificities of the LAMP assays were evaluated by comparison with the results of conventional nested PCR. The LAMP assays were highl...

Journal: :Intervirology 2009
Qiang Li Qing-Feng Zhou Chun-Yi Xue Jing-Yun Ma Dao-Zhong Zhu Yong-Chang Cao

A rapid detection assay based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been developed for detecting porcine reproductive and respiratory syndrome virus (PRRSV). The RT-LAMP assay utilized a set of six primers to amplify the open reading frame 6 (ORF6) of the PRRSV. The amplified products were analyzed by agarose gel electrophoresis or visualized by colorimet...

2013
Jie Li Peiyuan Wang Aiguo Zhang Ping Zhang Muhamd Alsarakibi Guoqing Li

Giardia lamblia is recognized as one of the most prevalent parasites in dogs. The present study aimed to establish a loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of G. lamblia from dogs. The fecal samples were collected and prepared for microscopic analysis, and then the genomic DNA was extracted directly from purified cysts. The concentration of DNA samp...

Journal: :iranian journal of parasitology 0
mohammad reza mahmoudi dept. of medical microbiology and parasitology, school of medicine, guilan university of medical sciences, rasht, iran. bahram kazemi dept. of parasitology and mycology, school of medicine, shahid beheshti university of medical sciences, tehran, iran. ali haghighi cellular and biology research centre, shahid beheshti university of medical sciences, tehran, iran. panagiotis karanis center for biomedicine and infectious diseases (cbid), qinghai academy for animal sciences and veterinary medicine, qinghai university, xining, china.

background : free-living amoebae such as acanthamoeba species may act as carriers of cryptosporidium and toxoplasma oocysts, thus, may play an important role in the water-borne transmission of these parasites. in the present study, a loop mediated isothermal amplification (lamp) method for detection of toxoplasma and a pcr assay were developed for investigation of acanthamoeba in environmental ...

Journal: :Japanese journal of infectious diseases 2012
Janisara Rudeeaneksin Supranee Bunchoo Sopa Srisungngam Pathom Sawanpanyalert Sawet Chamnangrom Atipa Kamolwat Porntip Thanasripakdeekul Tooru Taniguchi Chie Nakajima Yasuhiko Suzuki Benjawan Phetsuksiri

Definitive diagnosis of tuberculosis (TB) by conventional culture, followed by bacterial identification based on biochemical tests is time-consuming and tedious. Simple loop-mediated isothermal amplification (LAMP) specific for Mycobacterium tuberculosis complex, targeting the M. tuberculosis 16S ribosomal RNA gene, termed TB-LAMP, was evaluated as an alternative for rapid culture confirmati...

Journal: :The Indian Journal of Veterinary Sciences and Biotechnology 2023

The aim of the study was to compare Polymerase Chain Reaction (PCR) and Loop Mediated Isothermal Amplification (LAMP) assay for detection Salmonella in Chicken Meat. Food safety remains a major concern worldwide, with outbreaks foodborne illness resulting significant costs individuals food industry. genus is most prevalent group pathogens that are regularly isolated from food-producing animals ...

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