An outline of the chest X-ray findings on pyrophyllitosis was described in terms of working environments and its length of term in the dust. It was clarified that in pyrophyllitosis granular shadows were distributed in the upper and middle lung :fields, which used to indicate an accretion trend at an early stage. A fast development of :fibrosis in the lung was also assumed, from which standpoin...

Analysis of Env RNA expression in lung and airways Total RNA was isolated from lung, from trachea and from epithelial tissue scraped from the inside of the nose, by using a Polytron tissue homogenizer (Brinkmann) and Trizol RNA isolation reagent (Invitrogen). Samples were treated with DNase and with reverse transcriptase in the presence of a 3 0 Env primer; they were then subjected to 30 cycles...

Chemical investigations of the DCM extract from the roots of Endiandra anthropophagorum resulted in the isolation of a new cyclobutane lignan endiandrin B (1), together with the known natural products, endiandrin A (2), and (-)-dihydroguaiaretic acid (3). The structure of 1 was determined by extensive spectroscopic analyses, and confirmed by single crystal X-ray crystallography. Methylation of ...

Immune complexes isolated from pleural effusions of lung carcinomas were dissociated by ion exchange chromatography in the presence of 8 M urea. The antibodies thus obtained from 2 lung adenocarcinomas and 2 squamous cell carcinomas were assayed by indirect immunofluorescence against a variety of target cells in fresh suspensions, tissue cultures, and paraffin-embedded sections. Strong cytoplas...

Mesenchymal stromal cells (MSCs) reside in many organs including lung, as shown by their isolation from fetal lung tissues, bronchial stromal compartment, bronchial-alveolar lavage and transplanted lung tissues. It is still controversial whether lung MSCs can undergo mesenchymal-to-epithelial-transition (MET) and possess immune regulatory properties. To this aim, we isolated, expanded and chara...

OBJECTIVE To determine the lung age of patients with morbid obesity and to compare it with the chronological age of these patients, emphasizing the premature damage that morbid obesity does to the lungs. METHODS An open, prospective cross-sectional study comprising 112 individuals: 78 patients with morbid obesity (study group); and 34 non-obese individuals with normal pulmonary function resul...

Other authors reported the extraction of lung TM's soluble in 50% ammonium sulfate,but also demonstrated these antigens not to be cross-reactive with CEA (36, 37). Two antigens extractable with 0.85% NaCI solution were isolated by Mclntire and Sizaret (26). These antigens did not cross react with CEA, AFP, a-2 H-globulin, or f3-fetoprotein. Wat son et a!. (42, 43) utilized an immunoadsorption c...

BACKGROUND Isolation of methyl gamma linolenate from Spirulina platensis using flash chromatography and its apoptosis inducing effect against human lung carcinoma A- 549 cell lines. METHODS Gamma linolenic acid is an important omega-6 polyunsaturated fatty acid (PUFA) of medicinal interest was isolated from microalgae Spirulina platensis using flash chromatography system (Isolera system) as i...

Tissue resident mesenchymal stem cells (MSC) are important regulators of tissue repair or regeneration, fibrosis, inflammation, angiogenesis and tumor formation. Taken together these studies suggest that resident lung MSC play a role during pulmonary tissue homeostasis, injury and repair during diseases such as pulmonary fibrosis (PF) and arterial hypertension (PAH). Here we describe a technol...