A glucuronan lyase extracted from Sinorhizobium meliloti strain M5N1CS was purified to homogeneity by anion-exchange chromatography. The purified enzyme corresponds to a monomer with a molecular mass of 20 kDa and a pI of 4.9. A specific activity was found only for polyglucuronates leading to the production of 4,5-unsaturated oligoglucuronates. The enzyme activity was optimal at pH 6.5 and 50 d...

There are evidences showing that adverse impacts of aluminum (Al) on plants growth and development are associated with its effect on plasma membrane and cell wall. Influence of Al on peroxidation of membrane lipids, wall-bound phenolics, lignin, soluble phenolics and the activity of certain enzymes involved in metabolism of phenols, i.e., phenylalanine ammonia lyase (PAL) and polyphenol oxidase...

Aspergillus nidulans pectate lyase was purified from culture filtrates. The enzyme catalyzed a random eliminative cleavage reaction, had an apparent molecular weight of 40,000, and a pl of 4.2. Pectate lyase antisera were produced and used to identify pectate lyase clones in a cDNA expression library. Thirteen of 14 clones identified immunologically cross-hybridized. The identity of the single-...

Aspergillus nidulans pectate lyase was purified from culture filtrates. The enzyme catalyzed a random eliminative cleavage reaction, had an apparent molecular weight of 40,000, and a pl of 4.2. Pectate lyase antisera were produced and used to identify pectate lyase clones in a cDNA expression library. Thirteen of 14 clones identified immunologically cross-hybridized. The identity of the single-...

in this study, trichoderma harzianum bi was evaluated for its capability to reduce the incidence and severity of the root-knot nematode, meloidogyne javanica, and fusarium wilt, f. oxysporum f. sp. lycopersici, as causal agents of a complex disease of tomato in the laboratory and greenhouse conditions. initial in vitro studies revealed that the parasitism of m. javanica eggs by t. harzianum bi ...

Escherichia coli isocitrate lyase (EC 4.1.3.1.) can be phosphorylated in vitro by an ATP-dependent reaction. The enzyme becomes phosphorylated by an endogenous kinase when partially purified sonic extracts are incubated with [gamma-32P]ATP. Treatment of isocitrate lyase with diethyl pyrocarbonate, a histidine-modifying reagent, blocked incorporation of [32P]phosphate from [gamma-32P]ATP. The is...

the interaction between root-colonizing pseudomonas fluorescens strain cha0mcherry and three wheat cultivars (bolani, roushan, forno) was studied as a phenomenon to be employed in the control of wheat leaf rust disease (puccinia triticina). cha0mcherry was inoculated using seed treatment method. the application of cha0mcherry reduced the number of pustules on leaves via inducing systemic resist...

Influences of light and darkness on l-phenylalanine ammonia-lyase activity of black locust (Robinia pseudoacacia) seedlings were studied. Light initiates both an increase in enzyme activity (or enzyme synthesis) and a subsequent decrease in activity. These dual influences of light create cyclic changes in l-phenylalanine ammonia lyase activity in continuous light. The cyclic changes in activity...

Repression of the synthesis of isocitrate lyase by glucose and/or induction of the synthesis of isocitrate lyase by acetate in Phycomyces blakesleeanus were demonstrated. Both glycerol and ethanol failed to induce isocitrate lyase activity. Furthermore, glucose appeared to cause an in vivo catabolite inactivation of the derepressed enzyme. Isocitrate lyase was inactivated both reversibly and ir...

A lytic enzyme active against viable, intact staphylococci is released into culture fluids upon lysis of bacteriophage-infected Staphylococcus aureus PS53 cells. This enzyme, staphylococcal phage-associated lysin (PAL), was partially purified by ammonium sulfate precipitation and gel filtration through Sephadex G-200. PAL is optimally active at pH 6.5 and 30 C, and lytic activity is greatly enh...