Cell suspension cultures of tomato, Lycopersicon esculentum Mill. cv VFNT-Cherry, produce phytochelatins (poly[gamma-glutamylcysteinyl]glycines) when exposed to cadmium. The synthesis of these peptides is accompanied by a decline in cellular levels of glutathione. Buthionine sulfoximine, an inhibitor of glutathione synthesis, inhibits the sustained production of phytochelatins. However, phytoch...

Structural analogs of (-)-S-adenosyl+methionine (SAM), in which the heterocyclic base was modified, were used to initiate studies to elucidate the active site conformation of the enzyme l-aminocyclopropane-I-carboxylic acid (ACC) synthase, which was partially purified from Lycopersicon esculentum (tomato). These potential substrate analogs were screened for activity both as substrates and/or as...

The codons for the amino acid residues making up the proposed ATP-binding sites of the maize (Zea mays L.) endoplasmic reticulum and tomato (Lycopersicon esculentum) cytoplasmic Stress70 proteins were deleted from their respective cDNAs. The deletions had little effect on the predicted secondary structure characteristics of the encoded proteins. Both wild-type and mutant proteins were expressed...

Multiple shoots were induced from leaf explants of Lycopersicon esculentum cultivar MicroTom, within 20-25d, on MS medium supplemented with 8.9 microM benzylaminopurine (BAP)+1.14 microM indole-3-acetic acid (IAA). For rooting, elongated microshoots were excised and transferred onto MS medium supplemented with 4.9 microM indole-3-butyric acid (IBA). Well-developed roots and flower raceme were o...

Background Allergy to tomato (Lycopersicon esculentum) has been reported to occur in 3-16% of food allergic subjects. Tomato allergy is frequently manifested as OAS, urticaria and gastrointestinal symptoms. So far, three tomato allergens, Lyc e 1 (profilin), Lyc e 2 (invertase) and Lyc e 3 (nsLTP) are listed in the official IUIS allergen database. Although tomato allergy is frequently associate...

Francisco J. R am írez Departamento de Qui'mica-Ffsica, Facultad de Ciencias, Universidad de Malaga, 29071 Malaga, Spain and M artin J. Bukovac Department of Horticulture, Michigan State University, East Lansing, MI 48824, U.S.A. Z. Naturforsch. 48c, 666-668 (1993); received February 16/April 6,1993 Lycopersicon esculentum, Plant Cuticle, Nitrogen Oxide, FT-IR Analysis Nitrogen oxide binding to...

This study examines the types of structural information that can be gained by utilizing the scanning electron microscope (SEM) and a cryofracture technique to examine the host-parasite interaction. Roots of tomato, Lycopersicon esculentum cv. Marglobe, were cultured aseptically and inoculated with the root-knot nematode, Meloidogyne incognita. Twenty-four hours to four weeks after inoculation, ...

The green-fruited Lycopersicon hirsutum Humb. and Bonpi. accumulated sucrose to concentrations of about 118 micromoles per gram fresh weight during the final stages of development. In comparison, Lycopersicon esculentum Mill. cultivars contained less than 15 micromoles per gram fresh weight of sucrose at the ripe stage. Glucose and fructose levels remained relatively constant throughout develop...

The codons for the amino acid residues making up the proposed ATP-binding sites of the maize (Zea mays L.) endoplasmic reticulum and tomato (Lycopersicon esculentum) cytoplasmic Stress70 proteins were deleted from their respective cDNAs. The deletions had little effect on the predicted secondary structure characteristics of the encoded proteins. Both wild-type and mutant proteins were expressed...

Certain amino acids inhibit growth of tobacco (Nicotiana tabacum L. var. xanthi), tomato (Lycopersicon esculentum) carrot (Daucus carota), and soybean (Glycerine max L. co. Mandarin) cell cultures when nitrate or urea are the nitrogen sources but not when ammonia is the nitrogen source. These amino acids also inhibit development of nitrate reductase activity (NADH:nitrate oxidoreductase EC 1.6....