Oxidative damage to nuclear DNA is known to involve site-specific Fenton-type chemistry catalysed by redox-active iron or copper in the immediate vicinity of DNA. However, the presence of transition metals in the nucleus has not been shown convincingly. Recently, it was proposed that a major part of the cellular pool of loose iron is confined within the acidic vacuolar compartment [Yu, Persson,...

Introduction: Synaptosomes are sealed particles that contain mitochondria, cytoskeleton and vesicles which are necessary to synaptic events like neurotransmitter release and uptake in the nervous system. However, the effect of high and low temperatures on synaptosome membrane integrity and function during a time course after its extraction is less known. The purpose of this study was to assess ...

The role of myosin Is in endosomal trafficking and the lysosomal system was investigated in a Dictyostelium discoideum myosin I double mutant myoB-/C-, that has been previously shown to exhibit defects in fluid-phase endocytosis during growth in suspension culture (Novak et al., 1995). Various properties of the endosomal pathway in the myoB-/C- double mutant as well as in the myoB- and myoC- si...

background: the aim of present study is to evaluate the effect of polyvinyl pyrrolidone (pvp) routinly used during icsi procedure on sperm membrane integrity, and sperm chromatin status. materials and methods: this study was carried out on 21 semen samples from the infertile men referring to isfahan fertility and infertility center. the processed semen samples were divided into two portions. on...

Transfection of Mv1Lu mink lung type II alveolar cells with beta1-6-N-acetylglucosaminyl transferase V is associated with the expression of large lysosomal vacuoles, which are immunofluorescently labeled for the lysosomal glycoprotein lysosomal-associated membrane protein-2 and the beta1-6-branched N-glycan-specific lectin phaseolis vulgaris leucoagglutinin. By electron microscopy, the vacuoles...

the aim of this study was to investigate the effect of adding different levels of zinc sulfate in semen extender on bovine sperm quality during cryopreservation. semen was collected from 4 holstein bull twice a week and the proper samples were pooled. semen were allocated to four extender treatments consisted of 0, 50, 100, 150 µm of zinc sulfate in 5 replicates and then, were frozen. post-thaw...

Lysosomes and cathepsins, the most common hydrolytic enzymes in lysosomes, are available different models of cell death as necrosis apoptosis. This study investigated effect cathepsin B-selective inhibitor CA-074 on apoptotic necrotic neuronal death. Focal cerebral ischemia which has been formed by occlusion three-vessel consisting permanent middle artery temporary bilateral carotid for 60 minu...

(-)-Oleocanthal (OC), a phenolic compound present in extra-virgin olive oil (EVOO), has been implicated in the health benefits associated with diets rich in EVOO. We investigated the effect of OC on human cancer cell lines in culture and found that OC induced cell death in all cancer cells examined as rapidly as 30 minutes after treatment in the absence of serum. OC treatment of non-transformed...

The endocytic pathway is a system specialized for the uptake of compounds from the cell microenvironment for their degradation. It contains an arsenal of hydrolases, including proteases, which are normally enclosed in membrane-bound organelles, but if released to the cytosol can initiate apoptosis signaling pathways. Endogenous and exogenous compounds have been identified that can mediate desta...