Epithelial Na channels mediate Na reabsorption in the distal segments of the kidney, gut, and other organs (Garty and Palmer, 1997). They are vital to the control of blood volume and arterial blood pressure, as evi-denced by various forms of hypertension involving defects in the channels themselves or the renin–angio-tensin–aldosterone axis regulating them (Lifton, 1996). Several years ago the ...

The methods involved in determining the 850 kDa structure of the 30S ribosomal subunit from Thermus thermophilus were in many ways identical to those that are generally used in standard protein crystallography. This paper reviews and analyses the methods that can be used in phasing such large structures and shows that the anomalous signal collected from heavy-atom compounds bound to the RNA is ...

Epithelial Na channels mediate Na reabsorption in the distal segments of the kidney, gut, and other organs (Garty and Palmer, 1997). They are vital to the control of blood volume and arterial blood pressure, as evi-denced by various forms of hypertension involving defects in the channels themselves or the renin–angio-tensin–aldosterone axis regulating them (Lifton, 1996). Several years ago the ...

Summary Ribosome biogenesis is a fundamental multi-step cellular process that culminates in the formation of ribosomal subunits, whose production and modification are regulated by numerous factors. In this study, we analyze physiologic prokaryotic ribosome isolating bona fide pre-50S subunits from an Escherichia coli strain with factor ObgE, affinity tagged at its native gene lo...

P2X2 receptor channel, a homotrimer activated by the binding of extracellular adenosine triphosphate (ATP) to three intersubunit ATP-binding sites (each located ∼50 Å from the ion permeation pore), also shows voltage-dependent activation upon hyperpolarization. Here, we used tandem trimeric constructs (TTCs) harboring critical mutations at the ATP-binding, linker, and pore regions to investigat...

Many transcription factors, including the Escherichia coli cyclic AMP receptor protein (CRP), act by making direct contacts with RNA polymerase. At Class II CRP-dependent promoters, CRP activates transcription by making two such contacts: (i) an interaction with the RNA polymerase alpha subunit C-terminal domain (alphaCTD) that facilitates initial binding of RNA polymerase to promoter DNA; and ...

A wheat cultivar "Chinese Spring" chromosome substitution line CS-1S(l)(1B), in which the 1B chromosome was substituted by 1S(l) from Aegilops longissima, was developed and found to possess superior dough and breadmaking quality. The molecular mechanism of its super quality conformation is studied in the aspects of high molecular glutenin genes, protein accumulation patterns, glutenin polymeric...

A computational investigation of geometric parameters, infrared (IR) and Raman frequencies as well molecular Rayleigh scattering polycyclic aromatic hydrocarbons (PAHs), used models for subunits larger graphitic materials.

background: this study was aimed to assess the effects of angiotensin ii (ang ii) supplementation to the in vitro maturation (ivm) and in vitro culture (ivc) media of vitrified-warmed ovine oocytes on their developmental competence and expression of na+/k+/atpase in resulting embryos. methods: the slaughterhouse-derived immature oocytes (n=1069) were randomly distributed into four experimental ...