objective(s): in order to grow cells in a three-dimensional (3d) microenvironment, self-assembling peptides, such as puramatrix, have emerged with potential to mimic the extracellular matrix. the aim of the present study was to investigate the influence of the self-assembling peptide on the morphology, survival, proliferation rate, migration potential, and differentiation of human meningioma st...

background: uterine leiomyoma is the most common tumor among the female in the reproductive period. the first step to control the tumor is employing medical treatment. currently gnrh agonists are the most common drugs for medical therapy of uterine myoma.  climacteric-like symptoms and osteoporosis and recurrence of the size of tumor after gnrh agonists consumption are among the most common sid...

background: collagen-based three-dimensional (3d) in vitro systems have been introduced to study the physiological states of cells. as a biomolecule, collagen is usually extracted from terrestrial animals whilst aquatic animals like squid contain large amounts of collagen. methods: in order to make effective use of marine organisms, we selected persian gulf squid in 2015 to extract the required...

nowadays, cell -based and tissue engineered products have opened new horizons in treatment of incurable nervous system disorders. the number of studies on the role of schwann cells (sc) in treating nervous disorders is higher than other cell types. different protocols have been suggested for isolation and expansion of sc which most of them have used multiple growth factors, mitogens and fetal b...

Cells are active components in their environment and constantly adjusting their performance to improve extracellular milieu changing. This approach is reflected their tends of maintaining intracellular homeostasis. When the cells encounter stress or pathologic stimuli, they can undergo a new manner (adaptation) and new steady state for achieving viability and function. If the external stress is...

At high viable cell concentrations in large-scale mammalian cell culture processes, the accumulation of dissolved carbon dioxide (dCO(2), typically quantified as an equilibrium gas-phase concentration) becomes problematic as a result of low CO(2) removal rates at reduced surface-to-volume ratios. High dCO(2) concentrations have previously been shown to inhibit cell growth and product formation ...

Background Glutamine is an essential component in culture media for most of the mammalian cell lines. It is often used as an alternative source of energy by cells, along with glucose. Glutamine metabolism induces ammonia accumulation in cell culture. Elevated ammonia concentration above 2 mM has been shown to have negative impact on both cell growth and recombinant protein productivity [1-4]. I...

cells are active components in their environment and constantly adjusting their performance to improve extracellular milieu changing. this approach is reflected their tends of maintaining intracellular homeostasis. when the cells encounter stress or pathologic stimuli, they can undergo a new manner (adaptation) and new steady state for achieving viability and function. if the external stress is...

The amino acid and vitamin requirements of mammalian cells in culture have been carefully defined in the studies of Eagle and coworkers (1) who supplemented their medium with 5 per cent dialyzed serum. Knowledge concerning the growth requirements satisfied by the serum is completely lacking. In a preliminary report (2), evidence was presented for the protein nature of one of the serum growth fa...