On the basis of crystal structures of the pyrroloquinoline quinone (PQQ) dependent enzymes methanol dehydrogenase (MDH) and soluble glucose dehydrogenase (s-GDH), different catalytic mechanisms have been proposed. However, several lines of biochemical and kinetic evidence are strikingly similar for both enzymes. To resolve this discrepancy, we have compared the structures of these enzymes in co...

A cDNA clone, named ppcMDH-1 and covering a part of the coding region for the porcine cytosolic malate dehydrogenase (cMDH) mRNA, was isolated from a porcine liver cDNA library. Subsequently, mouse cMDH cDNA clones were isolated from mouse liver and heart cDNA libraries, using the ppcMDH-1 cDNA as a probe. The longest clone, named pmcMDH-5, was sequenced and the primary structure of the mouse c...

BACKGROUND The study aimed to determine the proportion of each priority level of patients, time of performance in each priority level, and the reliability of the South African Triage Scale (SATS) tool at the Mahalapye District Hospital - Emergency Department (MDH-ED), a setting where the majority of the nurses were not formally trained on the use of the SATS. METHODS This was a cross-sectiona...

The determinants of the thioredoxin (TRX)-dependent redox regulation of the chloroplastic NADP-malate dehydrogenase (NADP-MDH) from the eukaryotic green alga Chlamydomonas reinhardtii have been investigated using site-directed mutagenesis. The results indicate that a single C-terminal disulfide is responsible for this regulation. The redox midpoint potential of this disulfide is less negative t...

The effects of exogenously applied silicon (Si) on plant growth and the isoform patterns of NAD-dependent malate dehydrogenase (NAD-MDH, EC 1.1.1.37) and NADPdependent malic enzyme (NADP-ME, EC 1.1.1.40) were studied in two pea genotypes (cultivar Auralia and mutant line 1/220) subjected to differential Mn treatments (50, 200 and 500 μM Mn) under control conditions. The excess Mn inhibited plan...

The isozyme patterns and activities of six enzymes were determined in surgical biopsy samples of lung tumors and non-neoplastic pulmonary areas. Fetal lungs were also examined. No tissue differences were found in the isozyme pattern of hexokinase or alkaline phosphatase; small differences in pyruvate kinase isozyme proportions were observed. The tumors exhibited significant deviations with resp...

Abstract Aluminum (Al) is an important factor that limits plant growth under acidic soil conditions. However, several species developed distinct mechanisms limit the damage caused by high Al concentrations. In highbush blueberry (Vaccinium corymbosum), resistance are not fully understood. This study was designed to evaluate effect of toxicity on roots and leaves genotypes with contrasting [Star...

The objectives of this study were to determinethe interactions between two hepatic enzymes and some minerals in the liver of broiler chickens. The study was performed with male and female from 1 to 56 days of age of broiler chickens. Malic acid was added to the water and offered to chickens freely from the start to the end of the experiment with constant concentration. The treatments consisted ...

Formulations based on mineral fillers and polymeric matrices of different nature were studied to obtain halogen-free flame retardant compounds (HFFR) for cable applications. The work was carried out by comparing fire-retardant natural origin with synthetic ones available the market. As a reference, formulation micronized magnesium hydroxide (n-MDH, obtained from brucite) an ethylene-vinyl aceta...