abstraCt The chapter presents a case study following the activities of super users and local developers during the adoption of a new business application by an accounting firm in Scandinavia (referred to as the Company). The Company launched a program to train super users to help with this process because of the complexity of the new system, a generic, multipurpose application system replacing ...

It is well established that childhood maltreatment is an important predictor of marijuana use, but few studies have examined the mechanisms underlying this relationship. The current study examines marijuana motives as mediators of the relationship between childhood maltreatment and marijuana use in a sample of young adults. In addition, pathways from childhood maltreatment to emotion dysregulat...

In this paper, we discuss an architecture for integrating WWW applications that offer information and services in the same domain. At the center of this architecture exists a mediator, whose responsibilities are to interact with the user and to effectively exchange information with the underlying applications in order to accomplish the user’s task. The integration and interoperation of the exis...

Collective Intelligence takes advantage of collaboration, competition and integration. It often uses mixed groups of humans and computers to research in new unexplored ways. Ontologies, which are the main building block of the Semantic Web, are usually prepared by domain experts. We introduce a novel approach, which employs Collective Intelligence, towards building simple domain ontologies thro...

PICSEL is a project 1 aiming at building information servers over existing information sources that are distributed and possibly heteregeneous. The approach which has been chosen in PICSEL is to deene an information server as a knowledge-based mediator in which carin is used as the core logical formalism to represent both the domain of application and the contents of information sources that ar...

NK cells use surface NK receptors to discriminate self from non-self. The NK receptor ligand-binding domain (NKD) has been considered the sole regulator of ligand binding. Using a prototypic murine NK receptor, Ly49A, we show that the membrane proximal nonligand binding ecto-domain (the stalk region) is critical to ligand binding and signaling. The stalk region is required for receptor binding ...

DNA-damage signaling utilizes a multitude of posttranslational modifiers as molecular switches to regulate cell-cycle checkpoints, DNA repair, cellular senescence, and apoptosis. Here we show that RNF8, a FHA/RING domain-containing protein, plays a critical role in the early DNA-damage response. We have solved the X-ray crystal structure of the FHA domain structure at 1.35 A. We have shown that...

In somatic cells, phosphatidylinositol 3-kinase (PI3 kinase) is a critical intermediary in growth factor-induced mitogenesis. We have examined the role of this enzyme in meiotic maturation of Xenopus laevis oocytes. PI3 kinase activity was present in immunoprecipitates of the p85 subunit of PI3 kinase from immature oocytes and markedly increased following progesterone stimulation. Injection of ...

During ribosome-associated quality control, stalled ribosomes are split into subunits and the 60S-housed nascent polypeptides are poly-ubiquitinated by Listerin. How this low-abundance ubiquitin ligase targets rare stall-generated 60S among numerous empty 60S is unknown. Here, we show that Listerin specificity for nascent chain-60S complexes depends on nuclear export mediator factor (NEMF). The...