Background MicroRNAs are small and non-coding RNA molecules with approximately 22 nt in length that cause inhibition of translation or degradation of mRNA. MiR-155 is a kind of molecule with different functions, such as its role in proliferation, apoptosis, inflammation, differentiation, and immunity. One of its best known functions is apoptosis that affects on caspase-3 activity. The main aim...

Inhibitors of cyclooxygenase 2 (COX 2) and the mammalian target of rapamycin (mTOR) show direct and indirect antitumor effects in a variety of cancers. This study was designed to investigate the effects of the mTOR antagonist rapamycin and the COX 2 inhibitor celecoxib on cell growth and apoptosis in malignant melanoma. Cell proliferation was analysed by the cell proliferation ELISA BrdU and al...

Here, we have found that the natural isoquinoline alkaloid berberine induced apoptosis of SK-MEL-2 human melanoma cells, as assessed by an increase in sub-G1 phase (hipodiploidy) in flow cytometry analysis, which involved mitochondria and caspase activation, including caspases 3, 4, 7, 8, and 9. Berberine induced disruption of the mitochondrial transmembrane potential (ΔΨm), released cytochrome...

Previous studies have shown that histone deacetylase 6 (HDAC6) plays critical roles in many cellular processes related to cancer. However, its biological roles in the development of melanoma remain unexplored. Our aim was to investigate whether HDAC6 has a biological role in human melanoma development and to understand its underlying mechanism. In the present study, HDAC6 expression was up-regu...

Dynamic increase of a skin malignant melanoma (melanoma malignum) morbidity rate is observed in the world for several years. Though, this type of carcinoma comprises just 5% of all malignant skin cancers, it is responsible for a 75ñ80% of deaths caused by these tumors (1). Melanoma malignum is a cancer formed as a result of malignant transformation of melanin synthesizing cells ñ melanocytes. I...

Chemotherapy resistance and related defects in apoptotic signaling are crucial for the high mortality of melanoma. Effective drugs are lacking, also due to the fact that apoptosis regulation in this tumor is essentially not understood. The cytosine analogue ferropoptoside (N69), which contains an iron carbonyl complex, resulted in strong induction of apoptosis in melanoma cells starting already...

Preconditioning (PC) as a protective strategy against noxious insults can decline cell death and apoptosis. It has been approved that mitochondria play a key role in PC mechanism. The critical role of complex I (CI) in oxidative phosphorylation machinery and intracellular ROS production, particularly in the brain, accentuates its possible role in PC-induced neuroprotection. Here, differentiated...

Abstract The Provirus Integration sites for Moloney murine leukemia virus (Pim) kinase family has been studied extensively in tumorigenesis. We previously reported a key role of Pim2 negatively regulating T-cell responses to alloantigen. further investigated how regulates mediated anti-tumor using models adoptive cell therapy (ACT) and autologous hematopoietic transplantation (HCT). found that ...

Telomerase reverse transcriptase as the core component of telomere and most common non-coding region mutation gene in melanoma, may play a significant role development melanoma. The aim this study is to investigate anti-tumor effect human telomerase (hTERT) knockdown malignant melanoma its potential clinical value. We employed hTERT strategy detected expression target by transcriptase-quantitat...

Preconditioning (PC) as a protective strategy against noxious insults can decline cell death and apoptosis. It has been approved that mitochondria play a key role in PC mechanism. The critical role of complex I (CI) in oxidative phosphorylation machinery and intracellular ROS production, particularly in the brain, accentuates its possible role in PC-induced neuroprotection. Here, differentiated...