Ribonuclease A has been the most studied enzyme of the twentieth century. Work on ribonuclease A has endowed enzymology, as well as protein science, with many paradigms. This work continues to provide a framework for understanding protein structure – function relationships in atomic detail. In addition, knowledge gained by basic research is enabling the rapid deployment of ribonuclease A homolo...

We describe the impact of subtype differences on the seroreactivity of linear antigenic epitopes in envelope glycoprotein of HIV-1 isolates from different geographical locations. By computer analysis, we predicted potential antigenic sites of envelope glycoprotein (gp120 and gp4l) of this virus. For this purpose, after fetching sequences of proteins of interest from data banks, values of hydrop...

[1] We combine Synthetic Aperture Radar Interferometry (InSAR), tide gauge, and continuous GPS measurements to determine the spatial variation in vertical land motion (VLM) along the coast of the Los Angeles basin over the past decade, and to examine the impact of spatially variable VLM on relative sea level trends. By identifying radar scattering targets with long-term coherence we make height...

We have recent,ly determined the t,otal amino acid sequence of an extracellular nuclease of Staphylococcus aureus consisting of a single chain of 149 residues devoid of half-cystinelf 2 (see Fig. 1). This globular protein contains some regions of helical structure,3 can undergo completely reversible unfolding in a variety of denaturing solvents,4 and readily yields cryst’als suitable for X-ray ...

The preparation of optically active molecules needs a chiral auxiliary. It is important to use the minimum amount of this auxiliary, and from that point of view asymmetric catalysis is much more advantageous than stoichiometric asymmetric synthesis. Some homogeneous catalysts prepared from chiral complexes have become during the past few years a useful tool in asymmetric synthesis. The complexe...

Some 10 years ago, Canadian health care organizations came under extreme pressure to cut costs as a result of a $4 billion cut in federal transfer payments to the provinces (Merrifield, 2003). This led to facility closures, cutting of beds on hospital wards, and moves to outpatient and community-based care. Health care employees felt the impact as well, with health professionals reporting loss ...

Acknowledgements I would like to thank Baylor Fox-Kemper for not only advising me on my thesis, but also providing valuable information about pursuing a career in physical oceanography. Also, I would like to thank Mark Merrifield, who got me involved in this project and provided all the data collected from Waikiki. Finally, I am greatly appreciative of Brad Marston, for providing me with the op...

Functionalized chiral diazaphospholanes ligate to a variety of transition metals, yielding chiral, catalytically active, metal complexes. Previous work has established that amino acid derivatization of the carboxyl groups of (R,R)-N,N'-phthaloyl-2,3-(2-carboxyphenyl)-phenyl-3,4-diazaphospholane (1) yields phosphines that are excellent ligands for palladium-catalyzed asymmetric allylic alkylatio...

It is proposed that the scope of solid-phase peptide synthesis could be considerably broadened by attaching peptides to the solid-phase through functional side-chain groups rather than through the commonly used alpha-carboxyl groups. Side-chain attachment offers the use of a large variety of chemical linkages to solid supports. Attachment through the epsilon-amino group of the lysine residue to...

[reaction: see text] Flexible and versatile methods have been developed for the immobilization of chiral pyridinebis(oxazoline) ligands by covalent bonding to a solid support, either by grafting or by polymerization. Different spacers can easily be introduced to modulate the support-ligand distance and the electronic properties of the chiral ligand. As an example, 2,6-bis[(S)-4-isopropyloxazoli...