A penicillin-binding protein of molecular weight 76,000 inducible by beta-lactams was detected in methicillin-resistant Staphylococcus haemolyticus and Staphylococcus simulans. DNA from these strains hybridized to the mecA gene from Staphylococcus aureus; however, the chromosomal HindIII fragments containing the mecA genes were 3.4 kilobases in S. haemolyticus and 4.3 kilobases in S. simulans.

Cibacron blue F3GA (CB) was found to reduce the MIC of oxacillin for methicillin-resistant Staphylococcus aureus (MRSA). This effect was not observed with methicillin-susceptible S. aureus. CB alters the resistance level of MRSA through a factor(s) other than mecA-related products, major autolysins, or femAB products. The exact target(s) of CB in causing the effect is unknown.

AIM This study was conducted to determine the occurrence of methicillin-sensitive and Staphylococcus aureus (MSSA) methicillin-resistant S. aureus (MRSA) from bovine mastitis and to characterize them with respect to antibiotic resistance gene mecA. MATERIALS AND METHODS A total of 160 mastitic milk samples were screened for the presence of S. aureus. The presumptive positive isolates were con...

Methicillin-resistant Staphylococcus aureus (MRSA) have been found in various farm animal species throughout the world. Yet, methicillin-susceptible S. aureus (MSSA), methicillin-susceptible non-S. aureus (MS-NSA), and methicillin-resistant non-S. aureus (MR-NSA) were not investigated. Therefore, we persued to determine the diversity in their phenotypic virulence assay, phenotypic antimicrobial...

background and aims: staphylococcus aureus is one of the important pathogens around the world. the present investigation was carried out to study the distribution of staphylococcal cassette chromosome mec (sccmec) types and antibiotic resistance properties in methicillin-resistant staphylococcus aureus isolated from isfahan hospitals. materials and methods: a total of 250 clinical specimens wer...

aims: it is very important to detect the coagulase-negative staphylococci, which produce the hospital infections. being one of the most expensive and time-consuming stages before the polymerase chain reaction (pcr), dna extraction is one of the primary stages of pcr. then, it should be noticed that the elimination of the stage might save time and costs. the aim of this study was to compare two ...

results of total 60 s. aureus isolates, 51 (85%) were mrsa, and of 60 cons, 7 (11.66%) were mrcons. the results showed that the mic of chlorhexidine for all 120 isolates was 1-16 μg/ml. 15 (12.5%) isolates carried qaca/b gene, 26 (21.7%) carried qacc gene, and 38 (31.7%) carried smr gene. conclusions maintenance of mrsa isolates in the attendance of low amounts of antiseptics could result in th...

conclusions the rate of imlsb in s. aureus isolates is relatively high in the northwest iran. since isolates with inducible resistance may mutate and change to constitutive resistance, to prevent clinical treatment failure, d-test should be performed along with routine antibiotic susceptibility tests. in this study, ermc gene was the predominant genetic determinant for the expression of mlsb re...

Methicillin-resistant coagulase-negative staphylococci were isolated from the nares and skin of 1- to 8-week-old healthy chickens in three flocks from a farm. Isolation of methicillin-resistant coagulase-negative staphylococci was positive for 72 (25.7%) of the 280 chickens tested, with the frequency varying from 2.2 to 100% according to flock. A total of 45 appropriate isolates were selected a...

The rate of the onset of methicillin-resistant Staphylococcus epidermidis infections is increasing in Tunisia. We have isolated 32 S. epidermidis strains from dialysis fluid and needle cultures in dialysis service. The strains were identified by classic methods (colonial morphology, Gram staining, catalase test, coagulase test, and DNase test) as well as by API ID32 Staph. Susceptibilities to 1...