1. Glutamate dehydrogenase was inhibited by l-serine O-sulphate, beta-chloro-l-alanine, O-phospho-l-serine and beta-chloro-l-alanine methyl ester. With the exception of beta-chloro-l-alanine methyl ester which was an irreversible inhibitor, it was possible to reverse the inhibitory effects by dialysis. 2. Both NAD(+) and glutamate afford some protection against the inhibition due to the methyl ...

An earlier proposed endoperoxide intermediate in the biosynthesis of prostaglandins was detected in short-time incubations of arachidonic acid with the microsomal fraction of homogenates of sheep vesicular glands. Conversion of the endoperoxide into prostaglandin E(2) was stimulated by reduced glutathione but suppressed by p-mercuribenzoate and N-ethylmaleimide. The methyl ester of an unknown c...

We have isolated glutamic acid 5-methyl ester from an Escherichia coli protein that is involved in chemotaxis. The bacteria were first incubated with [methyl-“Hlmethionine under conditions which are known to result in methylation of the protein. The protein, isolated by gel electrophoresis, was then digested by successive treatment with three proteolytic enzymes. One of the products was [methyl...

Target-site and non-target-site herbicide tolerance are caused by the prevention of herbicide binding to the target enzyme and the reduction to a nonlethal dose of herbicide reaching the target enzyme, respectively. There is little information on the molecular mechanisms involved in non-target-site herbicide tolerance, although it poses the greater threat in the evolution of herbicide-resistant...

This work was to find out the dominant secondary metabolites derived from the fungus Trichoderma and to test them against skin cancer protein. The metabolites were extracted in 80% methanol from the fungal biomass of Trichoderma isolated from mangrove sediment. The crude methanol extract was purified and analysed for the secondary metabolites by GC-MS. Three predominant compounds (heptadecanoic...

This study evaluated the feasibility of using methyl ester prepared from waste cooking oil for electrical insulation in distribution transformers. Methyl is produced by a transesterification reaction between and methanol sodium hydroxide as catalyst. The physicochemical parameters breakdown voltage were determined. effects ageing on voltage, viscosity acid value also investigated. In addition, ...

A series of linear and macrocyclic peptides 3-12 were synthesized using 3,5-pyridinedicarboxylic acid (1) as starting material and screened for their antimicrobial, anti-inflammatory and anticancer activities. Bis-ester 3 was prepared from 1 and L-leucine methyl ester. Hydrazinolysis and hydrolysis of dipeptide methyl ester 3 with hydrazine hydrate or 1 N sodium hydroxide afforded compounds 4 a...

The objective of this greenhouse study was to evaluate tall fescue ( Festuca arundinacea ) seedling growth when seeded after herbicide application. Herbicide treatments included a nontreated control; 1.19 lb/acre 2,4-dichlorophenoxyacetic acid (2,4-D) + 0.32 methylchlorophenoxypropionic (MCPP) dicamba; 0.75 quinclorac; and 0.06 halosulfuron-methyl. Seeding done at 0, 3, 7, or 14 days applicatio...

Information on the impact of climate change growth weed species and their sensitivity to herbicides could help establish an efficient management strategy. Due excessive use acetolactate synthase (ALS)-inhibitor herbicides, resistance those is increasing globally. It is, thus, crucial find out whether efficacy these will in future due increase temperatures carbon dioxide concentration. Therefore...