Biosensors and more specifically biochips exploit the interactions between a target analyte and an immobilized biological recognition element to produce a measurable signal. Systems based on surface nucleic acid hybridization, such as microarrays, are particularly attractive due to the high degree of selectivity in the binding interactions. One of the drawbacks of this reaction is the relativel...

Enhancement of biochips and biochips scanners is a crucial issue in the actual industry that needs more sensitive and less expensive molecular analysis tools. In this aim, several points have to be re-examined: detector sensitivity, chip density, labeling. In this perspective, we have developed several different techniques based on soft lithography to pattern molecules. We also have developed a...

Complete blood cell counts (CBCs) are one of the most commonly ordered and informative blood tests in hospitals. The results from a CBC, which typically include white blood cell (WBC) counts with differentials, red blood cell (RBC) counts, platelet counts and hemoglobin measurements, can have implications for the diagnosis and screening of hundreds of diseases and treatments. Bulky and expensiv...

Microfabricated biochips are very efficient platforms for analysis of biologically important molecules such as DNA, RNA, enzymes, antibodies etc. These devices requires sample in micro/nano volume and produces faster and better results. For these reasons, we fabricated a simple, disposable microfluidic device for amperometric detection of DNA intercalated with methylene blue redox dye. The devi...

In this paper, we describe a simple and rapid method of fabricating hot embossing tools using polydimethylsiloxane (PDMS), which are then used to rapidly fabricate microchannels in polymethylmethacrylate (PMMA). A negative photoepoxy SU-8 or thick positive photoresist AZ4620 on silicon was used for molding during PDMS casting. Fabrication time of these PDMS tools was considerably less than thos...

This paper deals with the transition of the dielectric properties of cells as a marker of their permeabilization. A demonstration of the transition between positive and negative DiElectroPhoresis (DEP) as a reaction to electroporation pulses is achieved in a microfluidic device. Electropermeabilization of cell membrane is broadly used in cell biology for electrotransfert of genes and other mole...

We report Gold/Silica biochips for low cost biosensor devices. Firstly, the study of biochemical interactions on silica by means of Surface Plasmon Resonance (SPR) is presented. Secondly, Gold/Silica biochips are employed to reduce the strong quenching that occurs when a fluorophore is close to the gold surface. Furthermore, the control of the Silicalike thickness allows optimizing the distance...