Aberrant imprinting of the insulin-like growth factor II (IGF2) gene is a molecular hallmark of many tumors. Reactivation of the normally suppressed maternal allele leads to upregulation of the growth factor that promotes tumor growth. However, the mechanisms underlying the loss of imprinting (LOI) remain poorly defined. We examined the epigenotypes at the gene promoters that control IGF2 allel...

The insulin-like growth factor 2 gene (IGF2) is an imprinting gene, which mediates cell growth and apoptosis. The loss of imprinting (LOI) of IGF2 has been associated with the development of cancer. In the present study, loss LOI of IGF2 in lung cancer was analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in combination with DNA sequencing of samples c...

Insulin-like growth factor-2 (IGF-2) is a mitogen, growth and differentiation modulator for many cell types. It is mainly expressed during the prenatal development, and its activity strongly depends on the genomic imprinting. Genomic imprinting in the chorionic tissues of spontaneously eliminated human embryos has been studied on the model of 820-AG (Apa1) of the IGF-2 gene locus. Molecular and...

Colonies of eusocial Hymenoptera, such as ants, bees and wasps, have long been recognized as candidates for the study of genomic imprinting on the grounds of evolutionary conflicts that arise from close interactions among colony members and relatedness asymmetry owing to haplodiploidy. Although a general kinship theory of genomic imprinting predicts its occurrence under various circumstances of...

Combining straightforward molecular imprinting with orthogonal click chemistry and accessible fluorescent dyes, a modular approach has been developed to assemble turn-on optical sensors based on fluorescence resonance energy transfer in molecularly imprinted nanoparticles.

Macroporous polymer films with long-range uniformity and biotinyl-moiety selective recognition sites have been developed. A hierarchical molecular imprinting strategy afforded significant enhancements in quartz crystal microbalance (QCM) sensitivities towards biotinylated compounds.

The Arabidopsis MEDEA gene is required in maternal tissues to restrict cell proliferation in embryos. Molecular characterisation indicates that MEDEA encodes a Polycomb-group protein, particularly intriguing as MEDEA's maternal effects may be a consequence of genomic imprinting.

4-chloro-2-methylphenoxy acetic acid (mcpa) is one of the most important pesticides which is extensively used to control weeds in arable farmland. exposure to this compound occurs in general population and persons who occupationally handle it. the aim of this present work was the preparation of mcpa imprinting polymer and its application as a selective sample preparation technique for trace det...

Molecular Imprinting of Maltose Binding Protein: Tuning Protein Recognition at the Molecular Level Maya Zayats, Manu Kanwar, Marc Ostermeier, and Peter C. Searson* Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United States Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United Stat...

Genomic imprinting refersto a process that distinguishes paternally derived chromosomes from maternally derived chromosomes. Imprinting plays a critical role in gene expression, mammalian development, and human disease. However, the biological requirement for imprinting remains a mystery. In the first 2 columns on the topic, we will reviewhow imprinting was initially identified, present some hy...