The reverse-phase high-performance liquid chromatography elution data, expressed in glucose units, of 2-pyridylaminated oligosaccharides (PA-oligosaccharides) originated from N-glycosides of glycoproteins have been analyzed with multiple regression to obtain parameters of contribution ascribable to each monosaccharide unit. For the best results, the known PA-oligosaccharides were classified int...

The separation of compounds by chromatography is often followed by the need to separate the compounds from the stationary phase for quantification, rechromatography, or other analytical procedures. In thin-layer chromatography compounds are usually separated from the adsorbent by removal of the layer from the backing by scraping or suction, followed by elution of the compounds in columns or by ...

The near-infrared and visible wavelength spectrum of the water dimer is considered to be the major contributor to the so-called water continuum at these wavelengths. However, theoretical models of this spectrum require the simultaneous treatment of both monomer and dimer excitations. A model for treating this problem is proposed which is based upon a Franck-Condon-like separation between the mo...

OBJECTIVES To assess the performance of the Gram-negative-specific antibiotic temocillin in polymethylmethacrylate bone cement pre-loaded with gentamicin, as a strategy for local antibiotic delivery. METHODS Temocillin was added at varying concentrations to commercial gentamicin-loaded bone cement. The elution of the antibiotic from cement samples over a 2 week period was quantified by LC-MS....

DNA fragments that show a pattern of differential expression on differential display gels must be eluted from the gel matrix and reamplified to enable further analysis. Elution is usually achieved by heating excised gel slices in a small volume of either water or TE. Here we show that this elution step can adversely affect the ability of the eluted DNA to act as a template for PCR reamplificati...