Human (26) Brain ESC/iPSC* FPGC MPGC Oocyte 7 6 5 5 3 AdFront_Wen H1_Lis 10wE1_GuoF 10wE2_GuoF AmpMinus_Okae FcF53yNeun_Lis H9_Lis 11wE1_GuoF 19wE1_GuoF AmpPlus_Okae FcM55yNeun_Lis PBAT_Tang 17wE1_GuoF 19wE2_GuoF Rrbs_GuoHS FCN_Zill Ads_Lis* 7w_Tang 9w_Tang Hs1570_Zeng Ff_Lis* UW160_Gkoun UW165_Gkoun Hs1832_Zeng Imr90_Lis* Mfg12y_Lis Mouse (25) Brain ESC FPGC MPGC Oocyte 8 8 2 3 4 129_Xie 2line...

Drastic membrane reorganization occurs when mammalian sperm binds to and fuses with the oocyte membrane. Two oocyte protein families are essential for fertilization, tetraspanins and glycosylphosphatidylinositol-anchored proteins. The firsts are associated to tetraspanin-enriched microdomains and the seconds to lipid rafts. Here we report membrane raft involvement in mouse fertilization assesse...

Fatty acid oxidation is an important energy source for the oocyte; however, little is known about how this metabolic pathway is regulated in cumulus-oocyte complexes. Analysis of genes involved in fatty acid oxidation showed that many are regulated by the luteinizing hormone surge during in vivo maturation, including acyl-CoA synthetases, carnitine transporters, acyl-CoA dehydrogenases and acet...

The epigenetic factors causing competence differences between SN (surrounded nucleolus) and NSN (non-surrounded nucleolus) oocytes, the significance for the increased histone acetylation and methylation in SN oocytes, and whether chromatin configuration or histone modification determines oocyte competence, are unclear. This study has addressed these issues by using the ovary-holding (OH) stress...

this study investigated the effect of different harvesting techniques on the recovery rate and quality of bovine cumulus oocyte complexes (cocs) and their subsequent developmental competence including in vitro maturation, fertilization and blastocyst formation. the cocs were retrieved from slaughter ovaries using aspiration, slicing, or aspiration followed by slicing. the cocs were examined mic...

Vitrification method is widely used in oocyte cryopreservation for IVF but the birth rates are lower than that of the fresh oocyte. One of the known main reasons is structural instability of meiotic spindle and chromosome systems of mature oocyte. To get the best way for keeping competence of matured oocytes, we studied the best conditions for vitrification focused on equilibration times. The m...

During mammalian oocyte maturation there are marked changes in the distribution of mitochondria that supply the majority of the cellular ATP. Such redistribution of mitochondria is critical for oocyte quality, as oocytes with a poor developmental potential display aberrant mitochondrial distribution and lower ATP levels. Here we have investigated the dynamics of mitochondrial ATP production thr...