The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT) reduction assay is a frequently used and easily reproducible method to measure beta-amyloid (Abeta) toxicity in different types of single cell culture. To our knowledge, the influence of Abeta on MTT reduction has never been tested in more complex tissue. Initially, we reproduced the disturbed MTT reduction in neuron and ast...

A validation study on an in vitro skin irritation assay was performed with the reconstructed human epidermis (RhE) LabCyte EPI-MODEL24, developed by Japan Tissue Engineering Co. Ltd (Gamagori, Japan). The protocol that was followed in the current study was an optimised version of the EpiSkin protocol (LabCyte assay). According to the United Nations Globally Harmonised System (UN GHS) of classif...

The lymphocyte stimulation test (LST) is one of the most useful laboratory tests for the identification of allergy to a specific drug. The present study was conducted to examine utility of the LST using the [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) assay as a method for diagnosing drug allergy. The basic experimental conditions for the MTT assay were determined by u...

In vitro studies were carried out to evaluate the cytotoxic potential of the ethanolic and aqueous extracts of Luffa acutangula against human neuronal glioblastoma cells (U343) and human lung cancer cells (A549). Screening of these extracts was done using the brine shrimp lethality bioassay, MTT assay and SRB assay. The results showed the significant decrease of the viability of the cells in a ...

MTT (3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) reduction assay is a method that validates the viability of an active cell. Dehydrogenase in mitochondria converts yellow colored insoluble tetrazolium salt to purple colored watersoluble formazan. Sperm also have mitochondria in the midpiece, therefore sperm viability could be evaluated by MTT reduction assay. Several studi...

abstract boswellia has been widely used in traditional medicine for the treatment of different diseases such as cancer in iran. the aim of this study was to evaluate the effect of the gum methanol extract of boswellia thurifera on the viability and p53 gene expression of cultured breast cancer cells. the gum methanol extract was obtained in various concentrations using the maceration method. no...