Maize chlorotic mottle virus (MCMV) infects maize plants and causes significant losses in corn production worldwide. In this study, a real-time TaqMan RTPCR assay for efficient detection of MCMV was described. A pair of primers amplifying a 131-bp DNA fragment and a TaqMan probe was designed targeting the novel molecular marker based on MCMV genome analysis sequences. The assay designed was hig...

The mumps virus is a negative-strand RNA virus in the family Paramyxoviridae. Mumps infection results in an acute illness with symptoms including fever, headache, and myalgia, followed by swelling of the salivary glands. Complications of mumps can include meningitis, deafness, pancreatitis, orchitis, and first-trimester abortion. Laboratory confirmation of mumps infection can be made by the det...

A molecular genotyping assay for human immunodeficiency virus type 1 (HIV-1) circulating in Southeast Asia is difficult to design because of the high level of genetic diversity. We developed a multiplex real-time polymerase chain reaction (PCR) assay to detect subtype B, CRF01_AE, CRF33_01B, and three newly described circulating recombinant forms, (CRFs) (CRF53_01B, CRF54_01B, and CRF58_01B). A...

A new readout approach for the Hamiltonian Path Problem (HPP) in DNA computing based on the real-time polymerase chain reaction (PCR) is investigated. Several types of fluorescent probes and detection mechanisms are currently employed in real-time PCR, including SYBR Green, molecular beacons, and hybridization probes. In this study, real-time amplification performed using the TaqMan probes is a...

BACKGROUND The real-time polymerase chain reaction is currently the method of choice for quantifying nucleic acids in different DNA based quantification applications. It is widely used also for detecting and quantifying genetically modified components in food and feed, predominantly employing TaqMan and SYBR Green real-time PCR chemistries. In our study four alternative chemistries: Lux, Plexor...

要 旨 単 純 ヘ ル ペ ス ウ イ ル ス1型(HSV-1)お よ び2型(HSV-2)の 遺 伝 子 を 同時 に検 出 す るmultiplex real-time PCR法 を 開発 し た. HSV-1お よびHSV-2のgpD領 域 を標 的 と し,プ ラ イ マ ー お よ びTaqMan蛍 光 プ ロ ー ブ を 独 自 に 設 計 し た.検 出感 度 は15copies/tubeで,HSV-1お よ びHSV-2と も15か ら1.5×106 copies/tubeの 範 囲 で 定 量 が 可 能 で あ っ た.real-time PCR法 はPCR法 よ り も103倍 感 度 が 高 く,他 の ヘ ルペ ス属(CMV, EBV, HHV6, HHV-7お よ びVZV) DNAは 検 出 され なか っ た. 以 上 の結 果 か ら,今 回 開 発...

A novel real-time quantitative polymerase chain reaction (PCR) method using an attached universal template (UT) probe is described. The UT is an approximately 20 base attachment to the 5' end of a PCR primer, and it can hybridize with a complementary TaqMan probe. One of the advantages of this method is that different target DNA sequences can be detected employing the same UT probe, which subst...

BACKGROUND In this study, we evaluated the analytical performance and clinical potential of a one-step multiplex real-time PCR assay for the simultaneous detection of 14 types of respiratory viruses using the AdvanSure RV real-time PCR Kit (LG Life Sciences, Korea). METHODS Three hundred and twenty clinical specimens were tested with the AdvanSure RV real-time PCR Kit and conventional multipl...

A DNA computer readout approach based on real-time polymerase chain reaction (PCR) for the computation of Hamiltonian Path Problem (HPP) is the main interest in this research. Based on this methodology, real-time amplification of DNA template with TaqMan probes is performed with some modifications to realize the readout. The readout approach consists of two phases: real-time amplification in vi...