Amoebae of Naegleria fowleri nN68 became elongated flagellated cells 150 to 180 min after subculture to non-nutrient buffer. N. fowleri NF69 did not become elongated or flagellated under these conditions. Electron microscopic examination of N. fowleri confirmed that it is a typical eucaryotic protist with a distinct nuclear envelope and prominent nucleolus, numerous vacuoles and cytoplasmic inc...

Emerging Infectious Diseases • www.cdc.gov/eid • Vol. 14, No. 9, September 2008 1499 Most of the patients whose cases were diagnosed in Buenos Aires, including 5 who required hospitalization, were referred to Muñiz Hospital. Built a century ago, Muñiz Hospital comprises a number of independent pavilions surrounded by a spacious garden, where mosquitoes thrive, especially in summer. Thus, vector...

Naegleria fowleri, a pathogenic free-living amoeba, causes fatal primary amoebic meningoencephalitis (PAM) in humans and animals. The nfa1 gene (360 bp), cloned from a cDNA library of N. fowleri, produces a 13.1-kDa recombinant protein which is located on pseudopodia, particularly the food cup structure. The nfa1 gene plays an important role in the pathogenesis of N. fowleri infection. To exami...

Mice challenged intranasally with Naegleria fowleri died of primary amoebic meningoencephalitis. Mice given 30 mg of cyclophosphamide per kg of body weight daily for 10 days starting 2 days before challenge were protected. Neither cyclophosphamide nor serum from cyclophosphamide-treated mice inhibited N. fowleri in vitro. A metabolic product of cyclophosphamide, acrolein, inhibited growth and e...

Naegleria fowleri amoebae were lysed by adult fresh human serum, and their multiplication was inhibited in culture medium supplemented with 10% fresh human serum. Heat inactivation (56 degrees C, 30 min) of serum abrogated these lytic and inhibitory effects. Absorption of human serum with amoebae failed to reduce immunoglobulin levels, and no specific antibody was detected in untreated or treat...

Pumping station Pumping station 1 Filtered and chlorinated 1 0.5 27 + – From plant B to pumping station, 17.5 km Pumping station 2 Filtered and chlorinated 1 >2.5 26 – – From pumping station site 1 to pumping station site 2, 3.5 km Catchment areas: consumer households Household 1, underground storage tank Filtered and chlorinated 1 0.5 29 + – From pumping station site 2 to household 1, 2.03 km ...