The synthesis and subsequent modification of neurofilament (NF) polypeptides has been examined in pulse-chase experiments, using cultured chick spinal cord neurons. Fluorography of the [35S]methionine-labeled cytoskeletal proteins, after separation by two-dimensional gel electrophoresis, revealed that (a) the mid-size chicken NF protein, NF-M160, is synthesized as a smaller and more basic precu...

Neuroinflammation contributes to delayed (secondary) neurodegeneration following traumatic brain injury (TBI). Tumor necrosis factor receptor-associated 6 (TRAF6) signaling may promote post-TBI neuroinflammation, thereby exacerbating secondary injury. This study investigated the pathogenic functions of TRAF6 TBI in vivo and vitro . A rat model was established by air pressure contusion while lip...

A novel nanofiltration membrane bioreactor (NF-MBR) was constructed by integrating a loosely structured hollow fiber into an aerobic activated sludge system. In order to verify the feasibility of loose NF bioreactor, parallel experiments conventional ultrafiltration (UF-MBR) and NF-MBR were conducted investigate process performances, fouling characteristics. The showed relatively high permeabil...

To explore mechanisms controlling sonic hedgehog (Shh) expression in human cancers, we investigated regulation of Shh by the transcription factor NF-kappaB. We identify putative NF-kappaB binding sites in the human Shh promoter region that specifically bind NF-kappaB complexes. Further, NF-kappaB activation by tumor necrosis factor alpha (TNF-alpha) or p65 overexpression stimulates Shh promoter...

A new panel of greater than 300 monoclonal antibodies (mAbs) was prepared to the high, middle, and low Mr rat neurofilament (NF) subunits (NF-H, NF-M and NF-L, respectively). NF proteins were purified both from native, i.e., phosphorylated rat NFs and from enzymatically dephosphorylated rat NFs. The resulting mAbs were used to biochemically and immunochemically distinguish and characterize dist...

Recent reports suggest that NF-Y transcription factors are positive regulators of skotomorphogenesis in Arabidopsis thaliana. Three NF-YC genes (NF-YC3, NF-YC4, and NF-YC9) are known to have overlapping functions in photoperiod dependent flowering and previous studies demonstrated that they interact with basic leucine zipper (bZIP) transcription factors. This included ELONGATED HYPOCOTYL 5 (HY5...

This study seeks to analyse the relationship between needle-related fear (NF) and vaccination (VF), intention (VI), most commonly declared reasons for avoiding (RAV). The aim was expand information on subject in a pandemic context. Just before starting plan. A cross-sectional survey applied sample (N = 2,175) of adult Spanish citizens (37.60 ± 12.98 years), both sexes (women: 67.3%). Descriptiv...

The NF-Y gene family is a highly conserved set of transcription factors. functional factor complex made up trimer between NF-YA, NF-YB, and NF-YC proteins. While mammals typically have one for each subunit, plants often multigene families subunit which contributes to wide variety combinations functions. Soybean with an overexpression particular isoform GmNF-YC4-2 (Glyma.04g196200) in soybean cu...

The development of freestanding and binder-free electrode is an effective approach to perform the inherent capacity active materials promote mechanism study by minimizing interference from additives. Herein, we construct a cathode composed MoS3/PPy nanowires (NWs) deposited on porous nickel foam (NF) (MoS3/PPy/NF) through electrochemical methods, which can work efficiently as sulfur-equivalent ...

Developing high-performing oxygen evolution reaction (OER) electrocatalysts under high-current operation conditions is critical for future commercial applications of alkaline water electrolysis clean energy generation. Herein, we prepared a three-dimensional (3D) bimetallic oxyhydroxide hybrid grown on Ni foam (NiFeOOH/NF) by immersing (NF) into Fe(NO3)3 solution. In this unique 3D structure, t...