Bartholomew and Mittwer (1951) presented photographic evidence that the gram-positive staining area of bacterial cells could be fitted internally to the area demonstrated by the Dyar cell wall stain. Their conclusion was that the bacterial cell wall was not included in the grampositive area. Lamanna and Mallette (1954) explained Gram differentiation of intact cells on the basis that crystal vio...

Genital herpes simplex virus infection in women was studied by using conventional tissue culture (TC) virus isolation compared with short-term (24-h) TC on Lab-Tek chamber slides followed by fluorescent-antibody (FA) staining. Three different staining techniques were used after TC: (i) staining with biotin-avidin (TC-BA/FA), (ii) direct FA (TC-FA), and (iii) indirect FA. The TC-BA/FA method sho...

Chromoendoscopy with Lugol's staining remains the gold standard technique for detecting superficial SCC. An alternative technique, such as narrow-band imaging (NBI), for "optical staining" would be desirable, since NBI is a simpler technique and has no known complications. In this study, we compare NBI without magnification and chromoendoscopy with Lugol's staining for detecting high-grade dysp...

The C4d staining as a special tissue marker for humoral immunity has served criteria of pathological diagnosis for antibody-mediated rejection (ABMR) in Banff classification since 2003. However, the sensitivity and specificity of C4d staining have been questioned, and recently, C4d-negative ABMR has been more focused in renal allograft pathology. The aim of this study was to make certain of C4d...

Low-grade myofibroblastic sarcoma (LGMS) is a distinct mesenchymal myofibroblastic malignancy. The tumor may occur at a variety of sites, but is particularly associated with the head and neck. Of the two maxillary sarcomas that were analyzed in the present study, one was misdiagnosed as an inflammatory myofibroblastic tumor during pre-operative excision biopsy, and later presented with a differ...

tuberculose has increased in the recent years. use of a sensitive screening method, can be one of the influential parameters in reduction of pulmonary tuberculosis. current screening method in the laboratories is based on observation of bacilli in stained smear by ziel-neelsen procedure. the aim of this research was to study the pcr efficiency in all confirmed patients of hamadan province. twen...

OBJECTIVE Lynch syndrome is a hereditary cancer syndrome that increases the risks of colorectal and gynecologic malignancies such as endometrial and ovarian cancer. Studies have shown that mutations in mismatch repair genes (MSH2, MSH6, and MLH1) are associated with Lynch syndrome. The aim of our study was to estimate the value of MSH2, MSH6, and MLH1 immunohistochemistry based on family histor...

gingivitis is an inflammatory destructive disease mainly caused by microbial plaques. gram-positive and gram-negative bacilli or cocci have been isolated from dental plaque and gingivitis. this study was going to isolate and characterize the pathogenic bacteria from gingival of 100 patients with oral gingivitis. enriched and specific media used for isolation of bacteria. identification of isola...