An outbreak of Neospora caninum-associated abortion occurred in a South Carolina dairy wherein greater than 10% of the herd aborted over a 4-month period. Of the total number of cows at mid-late gestation, nearly 40% (28/71) aborted while the remaining 60% (43/71) gave birth to normal calves. Immunohistochemical examination of brain tissue from a subset of aborted fetuses confirmed N. caninum a...

BACKGROUND The aim of the study was to provide a point of reference to study the Neospora caninum infections in China. METHODS Genome DNA was extracted from the brains of aborted fetuses and specific PCR was performed with N. caninum Nc5-targeted specific primers. Fetal bovine brain tissues were homogenized and continuously cultured in Vero cells with double antibodies. The medium was replace...

Abstract Between December 2016 and April 2017, a spate of abortions occurred in closed dairy herd from the central eastern region Paraná, Brazil, which 75 cows aborted. To identify its cause, organ fragments were collected an aborted fetus for histopathology, blood samples stillborn, 4 fetuses, 9 farm dogs indirect fluorescent antibody technique (IFAT). These tests found multifocal non-suppurat...

Neospora caninum is now recognized as a major cause of abortion in cattle worldwide, but there is no report of N. caninum infection in cattle in India. Serum samples from 427 dairy cattle and 32 dairy water buffaloes from 7 organized dairy farms located in Punjab, India, were tested for N. caninum antibodies using a commercial monoclonal antibody-based competitive enzyme-linked immunosorbent as...

To determine whether deer can transmit Neospora caninum, brains of naturally infected white-tailed deer (Odocoileus virginianus) were fed to 4 dogs; 2 of these dogs shed oocysts. Oocysts from 1 of the dogs were tested by polymerase chain reaction and found to be positive for N. caninum and negative for Hammondia heydorni. The internal transcribed spacer 1 sequence of the new strain (designated ...

neospora caninum, an apicomplexan protozoan, is regarded as a major cause of abortion and stillbirth in cattle in countries world-wide. the ability to detection n.caninum in tissue samples can be a useful detection diagnostic tool for use in the study of the pathogenicity, immunoprophylaxis, and treatment of neospora infection. however, molecular biology is one of the most sensitive tools for d...

to determine the seroprevalence of anti-neospora caninum and anti-toxoplasma gondii antibodies indogs in iran and to investigate related risk factors to the infection, a study was conducted in chaharmahal vabakhtiari, isfahan and khoozestan provinces, locate in west central parts of iran. for this, 548 serum samples were collected from dogs during an 18 month period from july 2007 to february 2...

background: the aim of this study was to investigate the sero-prevalence of neospora caninum infec­tion in camelus dromedarius ; the most popular camel species in iran. methods:   totally, 310 serum samples were collected from camels in isfahan province during 2008 and tested in shahrekord university using indirect fluorescent antibody test (ifat) for se­rodiagnosis of anti- n. caninum igg anti...

Capybaras (Hydrochoerus hydrochaeris) are the largest rodents found in South America. The aim of the present study was to investigate the occurrence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in 170 free-living capybaras in a residential park area in Itu Municipality, São Paulo State, Brazil. Serum samples were tested by indirect fluorescent antibody test (IFAT) for T. gondi...

The main purpose of this investigation was to evaluate the presence of Neospora caninum infection in beef cattle from Hamedan province, Western Iran. Blood samples were collected from 514 industrial beef cattle and tested using ELISA. IgG antibodies against N. caninum were found in 102 (19.8%) sera. Significant differences were observed between seropositive cattle and age group (p=0.022). No si...