background: ovine babesiosis is the most important haemoparasitic tick-borne disease of small ruminants in iran caused by babesia ovis, b. motasi, and b. crassa . the aim of this study was to characterize the species of ovine babesia species isolated from different geographical region of iran. methods: one hundred fifty four blood samples collected from animals, which demonstrated the pale muco...

Deep sequencing was used to bring high resolution to the human cytomegalovirus (HCMV) transcriptome at the stage when infectious virion production is under way, and major findings were confirmed by extensive experimentation using conventional techniques. The majority (65.1%) of polyadenylated viral RNA transcription is committed to producing four noncoding transcripts (RNA2.7, RNA1.2, RNA4.9, a...

In the post-genomic era, the ability to synthesize any arbitrary DNA sequence is increasingly in demand. A bottleneck in current gene synthesis technologies is the associated cost, due primarily to the high cost of oligonucleotides synthesis and post-synthesis sequencing. In the present paper, an improved method for low-cost gene synthesis that combines dual asymmetrical PCR and overlap extensi...

conclusions in molecular epidemiology studies, nested pcr has higher sensitivity than pcr. results of this study support the colonization of p. jiroveci in patients receiving lung transplantation. patients who are carriers of p. jiroveci are at a higher risk of p. jiroveci pneumonia. results the genome of p. jiroveci was revealed by a 346 bp pcr product in the initial amplification and a 120 bp...

bovine leukemia virus (blv) is a retrovirus causing a chronic leukemia/lymphoma in cattle in many countries around the world. screening for antibodies by elisa has been the primary means of detecting the presence of this virus. it is not yet known if this assay will detect antibodies in all cattle during a concomitant bovine leukemia virus infection. the polymerase chain reaction (pcr) of the t...

in this study a nested-pcr assay was optimized for detection of two bvdv biotype of nadl strain. a part of 5' non-coding region of virus, 249 bp in size, was amplified in rt-pcr. pcr product was cloned in a ptz57r/t vector and sequencing results confirmed the specificity of the test. internal primers were designed and a 155 bp dna fragment was amplified in nested-pcr. the sensitivity of rt...

background: nematodes are among the most common and important parasites of man and animal. dna of a single worm can be used for several purposes, such as identification to the species, subspecies, strain and antihelmenthic resistance. dna extraction from a single small worm using traditional methods such as phenol extraction technique faces serious prob lems. methods: dna from 20 single haemonc...